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Product Pathways - Immunohistochemistry

SignalStain® DAB Substrate Kit #8059

Item# Description List Price Web Price Qty
8059P SignalStain® DAB Substrate Kit - 1 Kit $60.00
$54.00
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8059S SignalStain® DAB Substrate Kit - 1 Kit $200.00
$180.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Petite Kit Includes Quantity
SignalStain® DAB Diluent 1 x 12 ml
SignalStain® DAB Chromogen Concentrate 1 x 360 µl
Small Kit Includes Quantity
SignalStain® DAB Diluent 1 x 120 ml
SignalStain® DAB Chromogen Concentrate 1 x 3.6 ml

Description

The SignalStain® DAB Substrate Kit contains all of the necessary reagents to prepare a working solution of diaminobenzidine (DAB) for staining tissue sections. The DAB working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a brown reaction product.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145.


Method Overview

To prepare the DAB working solution, add 1 drop (30 μl) SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent and mix well before use. Working solutions are stable for up to 14 days when stored at 4ºC or up to 5 days when stored at room temperature (approximately 25ºC). While the solution may change color over that time period, it will have no effect on the quality of the staining.

*Please note that when using 8059P, a dropper for the chromogen concentrate is not included. Use a pipet to add 30 μl of SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent.

Important: DAB is a suspected carcinogen. Good laboratory practices should be followed, and appropriate protective equipment should be used when handling DAB, including gloves, protective eyeware, and a lab coat. Dispose in accordance with local regulations.

After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 1-10 minutes generally provides an acceptable staining intensity.

Wash slides in water for 5 minutes.

Counterstain, if desired, and mount coverslips with either a permanent mounting media or an aqueous-based mounting media. Please note, permanent mounting requires dehydration through ethanol and clearing through xylene.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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