New England Biolabs Canada
 
XP Monoclonal Antibody

Product Pathways - Tyrosine Kinase / Adaptors

Met (D1C2) XP® Rabbit mAb #8198

Item# Description List Price Web Price Qty
8198S Met (D1C2) XP® Rabbit mAb - 100 µl $433.00
$389.70
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8198T Met (D1C2) XP® Rabbit mAb - 20 µl $184.00
$165.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

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12630P SignalFire™ Plus ECL Reagent - 20 ml $116.00
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12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
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12757P SignalFire™ Elite ECL Reagent - 20 ml $123.00
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12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
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3077S Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb - 100 µl $480.00
$432.00
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3077T Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb - 20 µl $192.00
$99.00
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3121S Phospho-Met (Tyr1349) Antibody - 100 µl $433.00
$389.70
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3126L Phospho-Met (Tyr1234/1235) Antibody - 300 µl $1,025.00
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3126S Phospho-Met (Tyr1234/1235) Antibody - 100 µl $433.00
$389.70
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3129L Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb - 300 µl $1,028.00
$925.20
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3129S Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb - 100 µl $439.00
$395.10
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3133L Phospho-Met (Tyr1349) (130H2) Rabbit mAb - 300 µl $1,028.00
$925.20
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3133S Phospho-Met (Tyr1349) (130H2) Rabbit mAb - 100 µl $439.00
$395.10
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3133T Phospho-Met (Tyr1349) (130H2) Rabbit mAb - 20 µl $174.00
$99.00
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3135S Phospho-Met (Tyr1003) (13D11) Rabbit mAb - 100 µl $439.00
$395.10
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3135T Phospho-Met (Tyr1003) (13D11) Rabbit mAb - 20 µl $174.00
$99.00
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3148S Met (L41G3) Mouse mAb - 100 µl $372.00
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4560S Met Antibody - 100 µl $372.00
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5425S Normal Goat Serum - 10 ml $92.00
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6883P3 SignalFire™ ECL Reagent - 50 ml $43.00
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6883S SignalFire™ ECL Reagent - 500 ml $370.00
$333.00
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $109.00
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $203.00
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $646.00
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $326.00
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $92.00
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8112L SignalStain® Antibody Diluent - 100 ml $117.00
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8112S SignalStain® Antibody Diluent - 25 ml $57.00
$51.30
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8114P SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 1 ml $56.00
$50.40
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8114S SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) - 15 ml $525.00
$472.50
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8118S SignalSlide® Phospho-Met (Tyr1234/1235) IHC Controls - 1 Pack $200.00
$180.00
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9997S Tris Buffered Saline with Tween® 20 (TBST-10X) - 1000 ml $90.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human Endogenous 140, 170 Rabbit IgG
IP
IHC-Bond
IHC-P
IHC-F
IF-IC
F

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-Bond=IHC-Leica® Bond™, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry

Specificity / Sensitivity

Met (D1C2) XP® Rabbit mAb recognizes endogenous levels of total Met protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Met protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 (Met+), SK-BR-3 (Met-), and T-47D (Met-) cells using Met (D1C2) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Met (D1C2) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human metastatic lung carcinoma using Met (D1C2) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Met (D1C2) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Met (D1C2) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, MKN-45 (left) and T-47D (right), using Met (D1C2) XP® Rabbit mAb.


IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen MKN-45 xenograft using Met (D1C2) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 and T-47D cells using Met (D1C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of T-47D cells (blue) and HT-29 cells (green) using Met (D1C2) XP® Rabbit mAb.


Background

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).

  1. Cooper, C.S. et al. (1984) Nature 311, 29-33.
  2. Bottaro, D.P. et al. (1991) Science 251, 802-4.
  3. Bardelli, A. et al. (1997) Oncogene 15, 3103-11.
  4. Taher, T.E. et al. (2002) J Immunol 169, 3793-800.
  5. Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32.
  6. Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14.
  7. Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118.

Application References

  • Hyuga, S. et al. (2013) Planta Med 79, 1525-30. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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