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Product Pathways - Screening Technologies

O-GlcNAc MultiMab™ Rabbit mAb mix #82332

Item# Description List Price Web Price Qty
82332S O-GlcNAc MultiMab™ Rabbit mAb mix - 100 µl $424.00
$381.60
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity Isotype
W All Species Expected Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Homology

Species predicted to react based on 100% sequence homology: All Species Expected.

Protocols

Specificity / Sensitivity

O-GlcNAc MultiMab™ Rabbit mAb mix specifically recognizes endogenous levels of O-GlcNAc on proteins in β-O-glycosidic linkage to both serine and threonine.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a peptide containing serine-O-linked N-acetyl-glucosamine (O-GlcNAc).

Western Blotting

Western Blotting

Western blot analysis of HeLa cells untreated (-) or treated (+) with 10 μM Thiamet G (TMG) for 6 hours, using O-GlcNAc MultiMab™ Rabbit mAb mix with no blocking (left), blocking with free N-acetyl-D-glucosamine (center), or N-acetyl-D-galactosamine (right).

Western Blotting

Western Blotting

Western blot analysis of various cell lines with O-GlcNAc MultiMab™ Rabbit mAb mix.

Background

A distinct form of protein glycosylation, beta-linked N-acetyl-glucosamine (GlcNAc) moieties can be added to serine or threonine residues of proteins (1,2). This differs from other forms of glycosylation, as it typically is a single moiety rather than the complex branched sugars that are more commonly studied. It is thought that these modifications happen in a much more dynamic cycle more reminiscent of phosphorylation modifications (3). GlcNAc modified proteins are found in the cytoplasm and nucleus and are modulated by means of specific O-GlcNAc transferases (OGT) as well as GlcNAcase activity that can be inhibited using the Thiamet-G (TMG) inhibitor. Mass spectrometry analysis of this modification has been complicated due to the loss of the GlcNAc group during ionization and fragmentation, but methods and technologies such as electron transfer dissociation (ETD) are opening up new avenues to study these modifications. O-GlcNAc could play an important role in many cellular processes, including metabolism, growth, morphogenesis, apoptosis, transcription, and it may play a critical role in cancer.(4)

  1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
  2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
  3. Hart, G.W. and Akimoto, Y. (2009) Essentials of Glycobiology
  4. Capotosti, F. et al. (2011) Cell 144, 376-88.

Application References

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