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TAZ (E8E9G) Rabbit mAb #83669

Item# Description List Price Web Price Qty
83669S TAZ (E8E9G) Rabbit mAb - 100 µl $364.00
$327.60
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W Human, Mouse, Rat, Monkey Endogenous 55 Rabbit IgG
IP
IF-IC

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

TAZ (E8E9G) Rabbit mAb recognizes endogenous levels of total TAZ protein. This antibody does not cross-react with YAP.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to human TAZ protein. The epitope has been mapped to a region surrounding Pro49.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TAZ (E8E9G) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Expression levels of TAZ among cell lines are consistent with expectations based on publicly available bioinformatic databases.

IP

IP

Immunoprecipitation of TAZ protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TAZ (E8E9G) Rabbit mAb. Western blot analysis was performed using TAZ (D3I6D) Rabbit mAb #70148. Mouse anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF 10A cells (high-expressing) at low (left) and high-confluence (middle) versus SH-SY5Y cells (right, low-expressing) using TAZ (E8E9G) Rabbit mAb (green). Nuclei are labeled with DAPI #4083 (blue). Note that decreased nuclear localization of TAZ protein is seen in high-confluence MCF 10A cells.


Background

TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (1). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (1). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (2,3). TAZ is negatively regulated via phosphorylation by LATS1/2, core kinases in the Hippo signaling pathway that controls stem cell development, tissue growth and tumor development (4).

  1. Kanai, F. et al. (2000) EMBO J 19, 6778-91.
  2. Hong, J.H. et al. (2005) Science 309, 1074-8.
  3. Hong, J.H. and Yaffe, M.B. (2006) Cell Cycle 5, 176-9.
  4. Lei, Q.Y. et al. (2008) Mol Cell Biol 28, 2426-36.

Application References

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