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XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 594 Conjugate) #83707

Item# Description List Price Web Price Qty
83707S PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 594 Conjugate) - 100 µl $487.00
$438.30
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
84651S PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) - 100 µl $424.00
$381.60
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84651T PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) - 20 µl $181.00
$162.90
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity Isotype
IF-F Mouse Endogenous Rabbit IgG
IF-IC

Applications Key: IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total PD-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala242 of mouse PD-1 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in mouse cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) #84651.

Background

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).

  1. Ishida, Y. et al. (1992) EMBO J 11, 3887-95.
  2. Shinohara, T. et al. (1994) Genomics 23, 704-6.
  3. Nishimura, H. et al. (1999) Immunity 11, 141-51.
  4. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
  5. Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17.
  6. Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41.
  7. Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54.
  8. Thibult, M.L. et al. (2013) Int Immunol 25, 129-37.
  9. Dong, H. et al. (2002) Nat Med 8, 793-800.
  10. Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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