New England Biolabs Canada

Product Pathways -

FastScan™ Phospho-Tau (Ser416) ELISA Kit #90554

Item# Description List Price Web Price Qty
90554C FastScan™ Phospho-Tau (Ser416) ELISA Kit - 1 Kit $714.00
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at



The FastScan™ Phospho-Tau (Ser416) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Tau when phosphorylated at Ser416. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-Tau (Ser416) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-Tau (Ser416). Antibodies in kit are custom formulations specific to kit.

Cell-based assay

Cell-based assay

Figure 1. Phosphorylation of Tau at Ser416 in untreated mouse brain extracts can be detected using FastScan™ Phospho-Tau (Ser416) ELISA Kit #90554, but not in λ phosphatase-treated. The relationship between lysate protein concentration from untreated and λ phosphatase-treated mouse brain extracts and the absorbance at 450 nm using the ELISA Kit #90554 is shown in the upper figure. The corresponding western blots using phospho-Tau (Ser416) antibody (left panel) and Tau antibody (right panel) are shown in the lower figure. Collected mouse brain tissues were lysed and then either untreated or treated with λ phosphatase.


Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Toll Free: 1-800-387-1095

Fax: 1-800-563-3789

About NEBTerms of Sale Web Discount Shipping Information Web Site Disclaimer Privacy Policy NEB USA Cell Signaling Technology Sitemap
Contents ¬©New England Biolabs Ltd.  New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc.  New England Biolabs, Inc. is an ISO 9001 certified company.

Search another product:


Item has been added to the cart


Item has been added to the favourites