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FastScan™ Phospho-Tau (Ser416) ELISA Kit #90554

Item# Description List Price Web Price Qty
90554C FastScan™ Phospho-Tau (Ser416) ELISA Kit - 1 Kit $699.00
$629.10
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Protocols

Description

The FastScan™ Phospho-Tau (Ser416) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Tau when phosphorylated at Ser416. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-Tau (Ser416) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-Tau (Ser416). Antibodies in kit are custom formulations specific to kit.

Cell-based assay

Cell-based assay

Figure 1. Phosphorylation of Tau at Ser416 in untreated mouse brain extracts can be detected using FastScan™ Phospho-Tau (Ser416) ELISA Kit #90554, but not in λ phosphatase-treated. The relationship between lysate protein concentration from untreated and λ phosphatase-treated mouse brain extracts and the absorbance at 450 nm using the ELISA Kit #90554 is shown in the upper figure. The corresponding western blots using phospho-Tau (Ser416) antibody (left panel) and Tau antibody (right panel) are shown in the lower figure. Collected mouse brain tissues were lysed and then either untreated or treated with λ phosphatase.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

Application References

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