New England Biolabs Canada
 

Product Pathways - MAPK Signaling

Phospho-p90RSK (Ser380) Antibody #9341

Item# Description List Price Web Price Qty
9341S Phospho-p90RSK (Ser380) Antibody - 100 µl $433.00
$389.70
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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9326T p90RSK Antibody Sampler Kit - 1 Kit $453.00
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9333S RSK1 Antibody - 100 µl $372.00
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9335S Phospho-p90RSK (Ser380) (9D9) Rabbit mAb - 100 µl $439.00
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9343S RSK3 Antibody - 100 µl $372.00
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9344S Phospho-p90RSK (Thr359/Ser363) Antibody - 100 µl $433.00
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9346S Phospho-p90RSK (Thr573) Antibody - 100 µl $433.00
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9346T Phospho-p90RSK (Thr573) Antibody - 20 µl $174.00
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9347S RSK1/RSK2/RSK3 Antibody - 100 µl $372.00
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9348S Phospho-RSK3 (Thr356/Ser360) Antibody - 100 µl $433.00
$389.70
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9355S RSK1/RSK2/RSK3 (32D7) Rabbit mAb - 100 µl $372.00
$334.80
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9355T RSK1/RSK2/RSK3 (32D7) Rabbit mAb - 20 µl $159.00
$99.00
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Monkey Endogenous 90 Rabbit
IP

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Homology

Species predicted to react based on 100% sequence homology: Hamster, Dog.

Specificity / Sensitivity

Phospho-p90RSK (Ser380) Antibody detects endogenous levels of p90RSK only when phosphorylated at Ser380. This antibody does not recognize p90RSK phosphorylated at other sites. It shows some cross-reactivity with RSK2 and RSK3 when phosphorylated at the homologous serine residues.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser380 of human p90RSK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and C6 cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Ser380) Antibody (upper) or control RSK1/RSK2/RSK3 Rabbit mAb #9355 (lower).

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

Application References

  • Zaru, R. et al. (2007) Nat Immunol 8, 1227-35. Applications: Western Blotting.
  • Hans, A. (2001) . J. Biol. Chem. 276, 7258-7265. Applications: Western Blotting.
  • Delehedde, M. et al. (2000) . J. Biol. Chem. 275, 33905-33910. Applications: Western Blotting.
  • Majka, M. et al. (2000) . Blood 96, 4142-4151. Applications: Western Blotting.
  • Tomas-Zuber, M. et al. (2000) . J. Biol. Chem. 275, 23549-23558. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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