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Product Pathways - Apoptosis

Gasdermin D (L60) Antibody #93709

Item# Description List Price Web Price Qty
93709S Gasdermin D (L60) Antibody - 100 µl $364.00
$327.60
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat Endogenous 29, 53 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

Gasdermin D (L60) Antibody recognizes endogenous levels of total Gasdermin D protein. This antibody detects the N-terminal fragment of Gasdermin D upon proteolytic cleavage.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of mouse Gasdermin D protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Gasdermin D (L60) Antibody (upper) or β-Actin (D6A8) Rabbit mAb (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated (-) or treated (+) with TPA (12-O-Tetradecanolphorbol-13-Acetate) #4174 (50 ng/ml, 24 hr) followed by Lipopolysaccharides (LPS) #14011 (5 μg/ml, 6 hr), using Gasdermin D (L60) Antibody (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSMDC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSMDM-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).

  1. Kayagaki, N. et al. (2015) Nature 526, 666-71.
  2. Shi, J. et al. (2015) Nature 526, 660-5.
  3. Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.
  4. Aglietti, R.A. et al. (2016) Proc Natl Acad Sci U S A 113, 7858-63.
  5. Ding, J. et al. (2016) Nature 535, 111-6.
  6. Liu, X. et al. (2016) Nature 535, 153-8.
  7. Sborgi, L. et al. (2016) EMBO J 35, 1766-78.

Application References

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