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Product Pathways - Protein Translation

Phospho-4E-BP1 (Thr37/46) Antibody #9459

Item# Description List Price Web Price Qty
9459L Phospho-4E-BP1 (Thr37/46) Antibody - 300 µl $1,057.00
$951.30
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9459S Phospho-4E-BP1 (Thr37/46) Antibody - 100 µl $446.00
$401.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
1052S Phospho-4E-BP1 (Thr37/46) Blocking Peptide - 100 µg $472.00
$424.80
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12630P SignalFire™ Plus ECL Reagent - 20 ml $120.00
$108.00
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12630S SignalFire™ Plus ECL Reagent - 100 ml $519.00
$467.10
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12757P SignalFire™ Elite ECL Reagent - 20 ml $127.00
$114.30
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12757S SignalFire™ Elite ECL Reagent - 100 ml $558.00
$502.20
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2845S 4E-BP2 Antibody - 100 µl $383.00
$344.70
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2845T 4E-BP2 Antibody - 20 µl $163.00
$146.70
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2855L Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb - 300 µl $1,057.00
$951.30
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2855S Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb - 100 µl $446.00
$401.40
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2855T Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb - 20 µl $180.00
$162.00
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4923S Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb - 100 µl $446.00
$401.40
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4923T Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb - 20 µl $180.00
$162.00
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6883P3 SignalFire™ ECL Reagent - 50 ml $44.00
$39.60
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6883S SignalFire™ ECL Reagent - 500 ml $382.00
$343.80
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7074P2 Anti-rabbit IgG, HRP-linked Antibody - 100 µl $112.00
$100.80
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7074S Anti-rabbit IgG, HRP-linked Antibody - 1 ml $209.00
$188.10
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7074V Anti-rabbit IgG, HRP-linked Antibody - 5 ml $672.00
$604.80
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7216C PathScan® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Kit - 1 Kit $714.00
$642.60
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7727L Biotinylated Protein Ladder Detection Pack - 3250 µl $336.00
$302.40
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7727S Biotinylated Protein Ladder Detection Pack - 650 µl $94.00
$84.60
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9451L Phospho-4E-BP1 (Ser65) Antibody - 300 µl $1,057.00
$951.30
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9451S Phospho-4E-BP1 (Ser65) Antibody - 100 µl $446.00
$401.40
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9451T Phospho-4E-BP1 (Ser65) Antibody - 20 µl $180.00
$162.00
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9452L 4E-BP1 Antibody - 300 µl $928.00
$835.20
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9452S 4E-BP1 Antibody - 100 µl $383.00
$344.70
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9455L Phospho-4E-BP1 (Thr70) Antibody - 300 µl $1,057.00
$951.30
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9455S Phospho-4E-BP1 (Thr70) Antibody - 100 µl $446.00
$401.40
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9456S Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb - 100 µl $446.00
$401.40
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9644S 4E-BP1 (53H11) Rabbit mAb - 100 µl $383.00
$344.70
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9644T 4E-BP1 (53H11) Rabbit mAb - 20 µl $163.00
$146.70
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9741L Phospho-eIF4E (Ser209) Antibody - 300 µl $1,057.00
$951.30
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9741S Phospho-eIF4E (Ser209) Antibody - 100 µl $446.00
$401.40
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9741T Phospho-eIF4E (Ser209) Antibody - 20 µl $180.00
$162.00
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9918T Translational Control Antibody Sampler Kit - 1 Kit $636.00
$572.40
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9955T 4E-BP Antibody Sampler Kit - 1 Kit $636.00
$572.40
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VIEW COMPANION PRODUCTS HIDE COMPANION PRODUCTS
Application Dilution Species-Reactivity Sensitivity MW (kDa) Source
W Human, Mouse, Rat, Monkey Endogenous 15 to 20 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting

Protocols

Specificity / Sensitivity

Phospho-4E-BP1 (Thr37/46) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 of mouse 4E-BP1 and Thr46 of mouse 4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

  1. Pause, A. et al. (1994) Nature 371, 762-7.
  2. Brunn, G.J. et al. (1997) Science 277, 99-101.
  3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
  4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
  5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

Application References

  • Jiang, Y. P. et al. (2001) J. Biol. Chem. 276, 10943-10951. Applications: Western Blotting.
  • Kwiatkowski, D. J. et al. (2002) Hum. Mol. Genet. 11, 525-534. Applications: Western Blotting.
  • Yu, K. et al. (2001) Endocr. Relat. Cancer 8, 249-258. Applications: Western Blotting.
  • Ma, D. et al. (2009) J Cell Physiol 219, 31-44. Applications: Western Blotting.
  • Ohne, Y. et al. (2008) J Biol Chem 283, 31861-70. Applications: Western Blotting.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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