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Product Pathways - Companion Products

Protein A Agarose Beads #9863

Item# Description List Price Web Price Qty
9863P Protein A Agarose Beads - 200 µl $99.00
$89.10
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9863S Protein A Agarose Beads - 1 ml $200.00
$180.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Description

Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.

Product Specifications:

Bead Diameter: 45-165 micron per bead

Binding Capacity: 20 +/- 2 mg human IgG/ml

Directions For Use

Vortex tube briefly to resuspend the beads. Add 20 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.

IP

IP

Immunoprecipitation (IP) of COS-7 cell extracts using Ubc12 (D13D7) Rabbit mAb #5641 and Protein A Agarose Beads #9863. Western blot analysis was performed on the IP pellet (lane 1) and supernatant (lane 2) using Ubc12 (D13D7) Rabbit mAb #5641.

Application References

  • Caballero-Franco, C. et al. (2013) J Biol Chem 288, 23788-97. Applications: Companion Products.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


 

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