T7 Express Competent E. coli (High Efficiency)

Catalog # Concentration Size List Price Quantity Your Price
C2566H 20 x 0.05 ml $342.00
$307.80
C2566I 6 x 0.2 ml $260.00
$234.00
Catalog # Size List Price Your Price
C2566H 20 x 0.05 ml $342.00
$307.80
C2566I 6 x 0.2 ml $260.00
$234.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

High efficiency chemically competent E. coli cells suitable for T7 protein expression.

  • T7 RNA Polymerase in the lac operon - no λ prophage
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Free of animal products
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Chemically competent E. coli cells suitable for high efficiency transformation and protein expression.
Highlights
  • Enhanced BL21 derivative
  • T7 RNA Polymerase in the lac operon - no λ prophage
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-)
  • B Strain
  • Free of animal products
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  T7 Express Competent E. coli (High Efficiency) C2566HVIAL -80 20 x 0.05 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  T7 Express Competent E. coli (High Efficiency) C2566IVIAL -80 6 x 0.2 ml
Features
  • Most popular T7 expression strain
  • Protease deficient
Application Features

Transformation of a toxic mammalian clone into E. coli hostsTransformation of a toxic mammalian clone into E. coli hosts
A T7 expression plasmid and the same plasmid containing a gene encoding a toxic mammalian protein were transformed into each host. Comparison of the relative transformation efficiencies demonstrates that the T7 Express hosts provide the levels of control necessary for transformation of potentially toxic clones. BL21(DE3) could not be transformed with the toxic clone.
T7-controlled expression of a non-toxic protein in E. coli hostsT7-controlled expression of a non-toxic protein in E. coli hosts
A T7 expression plasmid containing a gene encoding an E. coli protein was transformed into each host, grown to 0.6 OD and induced for 3 hours. Comparison of soluble extracts from uninduced (-) and induced (+) cells shows superior control of basal expression in the T7 Express hosts while maintaining high levels of induced expression.
Effect of heat shock time on T7 Express competent E. coli transformation efficiencyEffect of heat shock time on T7 Express competent E. coli transformation efficiency
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds..
Effect of DNA incubation time on T7 Express competent E. coli transformation efficiencyEffect of DNA incubation time on T7 Express competent E. coli transformation efficiency
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection Working Concentration
Ampicillin 100 µg/ml
Carbenicillin 100 µg/ml
Chloramphenicol 33 µg/ml
Kanamycin 30 µg/ml
Streptomycin 25 µg/ml
Tetracycline 15 µg/ml

Shipping Notes

  • Ships on dry ice

Antibiotic Resistance

  • nit


Materials Sold Separately
Notes
  • CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
  • STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
Additional Citations
  • Anton, B.P., Fomenkov, A., Raleigh, E.A. and Berkmen, M. (2016) Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents Genome Announc Mar 31;4(2),PubMedID: 27034504, DOI: 10.1128/genomeA.00230-16.
Publications
  • Anton, B.P., Fomenkov, A., Raleigh, E.A. and Berkmen, M. (2016). Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents Genome Announc. Mar 31;4(2),PubMedID: 27034504, DOI: 10.1128/genomeA.00230-16.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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