Product Class: Kit

Quick Blunting™ and Quick Ligation™ Kits
Item# Description List Price Web Price Qty
E0542L Quick Blunting and Quick Ligation Kits - 100 rxns $916.00
$824.40
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E0542S Quick Blunting and Quick Ligation Kits - 20 rxns $229.00
$206.10
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Product Introduction

NEB offers the Quick Blunting™ and Quick Ligation Kits™ together as a bundle at a value price.

  • The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. 
  • The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).

Product Information

Description

NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.

The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).

Kit Components

The following reagents are supplied with this product:

Store at (°C) Concentration
10X Blunting Buffer -20 10 X
Quick Ligation Reaction Buffer -20 2 X
Blunting Enzyme Mix -20 20 reactions
Quick Ligation™ Kit -20
Deoxynucleotide Solution Mix (1 mM) -20 1 mM
Product Categories:
DNA Ligases Products
Applications:
Phosphorylation (Kinase),
Blunting,
Cloning Ligation
,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB

Properties & Usage

Product Notes

  1. Please note storage conditions for individual products.

Protocols, Manuals & Usage

Protocols

  1. Blunting protocol for NEB PCR Cloning Kit
  2. Protocol for the Quick Blunting Kit (E1201)
  3. Quick Ligation Protocol (M2200)
  4. Transformation Protocol

Usage & Guidelines

  • Tips for Maximizing Ligation Efficiencies

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

Tools & Resources

Web Tools

Faqs & Troubleshooting

FAQs

  1. Will the PreCR Repair Mix blunt the ends of the DNA?

Troubleshooting

  • Troubleshooting Tips for Ligation Reactions

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.