The NEBNext® Fast DNA DNA Library Prep Set for Ion Torrent® enables library preparation from pre-sheared DNA, with a streamlined, high-performance library prep workflow.
If enzymatic shearing of DNA is preferred, a version of the kit that includes enzymatic fragmentation reagents is available.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E6270L | -20 |
NEBNext® DNA Library Adaptors for Ion Torrent™ | E6274AAVIAL | -20 | 1 x 1 ml | ||
NEBNext End Repair Reaction Buffer | E6272AAVIAL | -20 | 1 x 0.3 ml | 10 X | |
T4 DNA Ligase | E6275AAVIAL | -20 | 1 x 0.3 ml | ||
NEBNext End Repair Enzyme Mix | E6271AAVIAL | -20 | 1 x 0.15 ml | ||
TE Buffer | E6293AAVIAL | -20 | 1 x 3.3 ml | 0.1 X | |
NEBNext DNA Library Primers for Ion Torrent™ | E6291AAVIAL | -20 | 1 x 0.5 ml | ||
T4 DNA Ligase Buffer for Ion Torrent™ | E6276AAVIAL | -20 | 1 x 0.5 ml | ||
Bst 2.0 WarmStart® DNA Polymerase | E6277AAVIAL | -20 | 1 x 0.05 ml | ||
NEBNext Q5 Hot Start HiFi PCR Master Mix | E6629AAVIAL | -20 | 2 x 1.25 ml | 2 X |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the NEBNext® Fast DNA Library Prep Set for Ion Torrent™ for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.
NEBNEXT®, NEW ENGLAND BIOLABS®, Q5® and WARMSTART® are registered trademarks of New England Biolabs, Inc.