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NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module

Catalog # Concentration Size List Price Quantity Your Price
E6421L 96 reactions $3,516.00
$3,164.40
E6421S 24 reactions $1,034.00
$930.60
Catalog # Size List Price Your Price
E6421L 96 reactions $3,516.00
$3,164.40
E6421S 24 reactions $1,034.00
$930.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

How low can you go?

NEBNext® is now available for single cell and ultra-low RNA inputs!

This unique cDNA synthesis and amplification workflow meets the demand for a highly sensitive, yet robust method that consistently ultimately enables generation of high quality sequencing data from single cell or ultra-low input RNA.

  • Generate the highest yields from single cells, or as little as 2 pg - 200 ng RNA
  • Experience unmatched detection of low abundance transcripts
  • Rely on consistent transcript detection for a wide range of input amounts and sample types
  • Observe uniform transcript coverage, regardless of input amount or sample type
  • Use with a variety of RNA inputs, including cultured or primary cells, or total RNA
  • Save time with a fast, streamlined workflow, minimal handling steps and hands-on time

Please note that this module contains only reagents for cDNA synthesis and amplification. The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB #E6420) includes reagents for DNA library construction.

The NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA. This unique workflow enables generation of high quality sequencing data from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.

Features:

  • The highest yields from single cells, or 2 pg - 200 ng RNA
  • Input can be cultured or primary cells, or total RNA
  • Single-tube protocol from cell lysis to cDNA
  • Fast, streamlined, automation-friendly workflow, with minimal handling steps and hands-on time

Download extensive performance data in our technical note.

Figure 1: NEBNext Single Cell/Low Input workflow




Figure 2: Higher cDNA yields with the NEBNext Single Cell/ Low Input cDNA Synthesis & Amplification Module




Sequencing libraries were generated from HeLa, Jurkat and M1 single cells, or 10 pg of Universal Human Reference (UHR) RNA (Agilent® #740000) with recommended amounts of ERCC RNA Spike-In Mix I (Thermo Fisher Scientific® #4456740). The NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module or the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Clontech® # 634891) were used. cDNA libraries were amplified using the number of PCR cycles shown. Error bars indicate standard deviation for 8-21 replicates.

 

View extensive performance data in our technical note.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® Single Cell RT Primer Mix E6422AVIAL -20 1 x 0.048 ml
  NEBNext® Single Cell RT Buffer E6423AVIAL -20 1 x 0.12 ml
  NEBNext® Template Switching Oligo E6424AVIAL -20 1 x 0.024 ml
  NEBNext® Single Cell RT Enzyme Mix E6425AVIAL -20 1 x 0.048 ml
  NEBNext® Single Cell cDNA PCR Master Mix E6426AVIAL -20 1 x 1.2 ml
  NEBNext® Single Cell cDNA PCR Primer E6427AVIAL -20 1 x 0.048 ml
  NEBNext® Cell Lysis Buffer E6428AVIAL -20 1 x 0.048 ml
  Murine RNase Inhibitor E6429AVIAL -20 1 x 0.024 ml
  NEBNext® Bead Reconstitution Buffer E6430AVIAL -20 1 x 1.08 ml
  TE Buffer E6432AVIAL -20 1 x 1.2 ml
  Nuclease-free Water E6433AVIAL -20 1 x 1.44 ml
  NEBNext® Single Cell RT Primer Mix E6422AAVIAL -20 1 x 0.192 ml
  NEBNext® Single Cell RT Buffer E6423AAVIAL -20 1 x 0.48 ml
  NEBNext® Template Switching Oligo E6424AAVIAL -20 1 x 0.096 ml
  NEBNext® Single Cell RT Enzyme Mix E6425AAVIAL -20 1 x 0.192 ml
  NEBNext® Single Cell cDNA PCR Master Mix E6426AAVIAL -20 1 x 4.8 ml
  NEBNext® Single Cell cDNA PCR Primer E6427AAVIAL -20 1 x 0.192 ml
  NEBNext® Cell Lysis Buffer E6428AAVIAL -20 1 x 0.192 ml
  Murine RNase Inhibitor E6429AAVIAL -20 1 x 0.096 ml
  NEBNext® Bead Reconstitution Buffer E6430AAVIAL -20 1 x 4.32 ml
  TE Buffer E6432AAVIAL -20 1 x 4.8 ml
  Nuclease-free Water E6433AAVIAL -20 1 x 5.76 ml
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

DataCards
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.

For additional information or to inquire about commercial use, please contact busdev@neb.com.

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