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NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

Catalog # Concentration Size List Price Quantity Your Price
E7330L 96 reactions $6,338.00
$5,704.20
E7330S 24 reactions $1,863.00
$1,676.70
Catalog # Size List Price Your Price
E7330L 96 reactions $6,338.00
$5,704.20
E7330S 24 reactions $1,863.00
$1,676.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
 
Please note: index primers for multiplexing are not included in this kit. This version of the kit is intended for use with home brew primers. See FAQ for more information on how to design indexing i7 primer.

  • Minimized adaptor-dimer contamination
  • High yields
  • Input RNA can be Total RNA
  • Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs


The NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next-generation sequencing on the Illumina platform. The novel workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-diversity libraries.
 
Please note that this kit, the NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible), enables use with your own barcode system. This kit contains adaptors but does not include multiplex primers. If index primers are required, kits NEB #E7300, NEB #E7580 or NEB #E7560 should be ordered instead.
 
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a small RNA library on the Illumina sequencing platform.
 

Small RNA Library Preparation Workflow for Illumina
Small RNA Library Preparation Workflow for Illumina

GenomeWeb article reviews NEBNext Small RNA Kits.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext 3´ Ligation Reaction Buffer E7301AVIAL -20 1 x 0.24 ml
  Murine RNase Inhibitor E7308AVIAL -20 1 x 0.024 ml
  NEBNext 5´ Ligation Reaction Buffer E7304AVIAL -20 1 x 0.024 ml
  NEBNext 5´ Ligation Enzyme Mix E7305AVIAL -20 1 x 0.06 ml
  LongAmp™ Taq 2X Master Mix E7309AVIAL -20 1 x 1.2 ml
  Gel Loading Dye, Blue E6138AVIAL -20 1 x 0.2 ml 6 X
  Linear Acrylamide E7325AVIAL -20 1 x 0.048 ml
  Nuclease-free Water E7327AVIAL -20 1 x 5 ml
  Quick-Load pBR322 DNA-MspI Digest E7323AVIAL -20 1 x 0.24 ml
  DNA Gel Elution Buffer E7324AVIAL -20 1 x 12 ml
  TE Buffer E7326AVIAL -20 1 x 0.48 ml
  NEBNext Index 1 Primer for Illumina E7329AVIAL -20 1 x 0.12 ml 10 µM
  NEBNext 3´ SR Adaptor for Illumina E7332AVIAL -20 1 x 0.024 ml
  NEBNext SR RT Primer for Illumina E7333AVIAL -20 1 x 0.024 ml
  NEBNext SR Primer for Illumina E7310AVIAL -20 1 x 0.06 ml
  NEBNext 5´ SR Adaptor for Illumina E7328AVIAL -20 1 x 1,350 pmol
  NEBNext First Strand Synthesis Reaction Buffer E7334AVIAL -20 1 x 0.192 ml
  ProtoScript II Reverse Transcriptase E7355AVIAL -20 1 x 0.024 ml
  NEBNext 3´ Ligation Enzyme Mix E7288AVIAL -20 1 x 0.072 ml
  NEBNext 3´ Ligation Reaction Buffer E7301AAVIAL -20 1 x 0.96 ml
  Murine RNase Inhibitor E7308AAVIAL -20 1 x 0.096 ml
  NEBNext 5´ Ligation Reaction Buffer E7304AAVIAL -20 1 x 0.096 ml
  NEBNext 5´ Ligation Enzyme Mix E7305AAVIAL -20 1 x 0.24 ml
  LongAmp™ Taq 2X Master Mix E7309AAVIAL -20 4 x 1.2 ml
  Gel Loading Dye, Blue E6138AAVIAL -20 1 x 1 ml 6 X
  Linear Acrylamide E7325AAVIAL -20 1 x 0.192 ml
  Nuclease-free Water E7327AAVIAL -20 1 x 20 ml
  Quick-Load pBR322 DNA-MspI Digest E7323AAVIAL -20 1 x 0.96 ml
  DNA Gel Elution Buffer E7324AAVIAL -20 1 x 48 ml
  TE Buffer E7326AAVIAL -20 1 x 1.92 ml
  NEBNext Index 1 Primer for Illumina E7329AAVIAL -20 1 x 0.48 ml 10 µM
  NEBNext 3´ SR Adaptor for Illumina E7332AAVIAL -20 1 x 0.096 ml
  NEBNext SR RT Primer for Illumina E7333AAVIAL -20 1 x 0.096 ml
  NEBNext SR Primer for Illumina E7310AAVIAL -20 1 x 0.24 ml
  NEBNext 5´ SR Adaptor for Illumina E7328AVIAL -20 1 x 1,350 pmol
  NEBNext First Strand Synthesis Reaction Buffer E7334AAVIAL -20 1 x 0.768 ml
  ProtoScript II Reverse Transcriptase E7355AAVIAL -20 1 x 0.096 ml
  NEBNext 3´ Ligation Enzyme Mix E7288AAVIAL -20 1 x 0.288 ml

Properties & Usage

Materials Required but not Supplied

3 M Sodium Acetate, pH 5.5
100% Ethanol
80% Ethanol
Corning®, Costar®, Spin-X® Centrifuge Tube Filters (Cellulose Acetate Filters) (Sigma Aldrich # CLS8162)
Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Size Selection Materials
for gel size selection:
6% Novex® TBE PAGE gel 1.0 mM
10-well (Life Technologies, Inc. #EC6265BOX)
SYBR® Gold Nucleic Acid Gel Stain (Life Technologies, Inc. #S-11494)
RNase-free Disposable Pellet Pestles® (Kimble Kontes Asset Management, Inc. #749521-1590)
Dry Ice/Methanol Bath or –80°C freezer

for bead selection:
Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)

for Pippin Prep™ selection:
3% Agarose Dye Free Gel (Sage Science #CDF 3010)

QIAquick® PCR Purification Kit (Qiagen® #28104)
Bioanalyzer® (Agilent Technologies, Inc.)

Notes
  • Kit Optimization

    The components of this kit were collectively optimized with FirstChoice®Human Brain Reference RNA (Life Technologies, Inc. #AM7962).

    High quality total RNA should be used as starting material. An RNA IntegrityNumber (RIN) > 7 is recommended.

    Assess the quality and quantity of your sample using the Agilent 2100Bioanalyzer using an Agilent RNA 6000 Nano Chip.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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