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NEBNext® Ultra™ RNA Library Prep Kit for Illumina®

Catalog # Concentration Size List Price Quantity Your Price
E7530L 96 reactions
Please Inquire
Catalog # Size List Price Your Price
E7530L 96 reactions
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
This original version of the Ultra™ RNA Library Prep Kit enables non-directional RNA library preparation in a streamlined workflow. However, we now recommend use of the more recent NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770), which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts. 

 

The NEBNext® Ultra™ RNA Library Prep Kit for Illumina® contains reagents for preparation of non-directional RNA libraries for Illumina sequencing. Please note that adaptors, primers, rRNA depletion reagents and poly(A) mRNA isolation reagents are not included in the kit and are available separately.

Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a library on the Illumina sequencing platform. 

NEBNext Ultra™ RNA provides even sequence coverage across transcripts for a wide range of inputs
NEBNext Ultra™ RNA provides even sequence coverage across transcripts for a wide range of inputs

10 ng, 100ng or 1 μg of Total RNA (Universal Human Reference RNA, Agilent #740000) was used as input for libraries, following the NEBNext Ultra™ RNA protocol and using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries were sequenced (single end reads) on the Illumina platform (GAIIx). Good coverage across expressed transcript was obtained for each input amount.

Ultra Non-directional RNA Library Preparation for IlluminaNon-Directional
Features
  • Low input amounts (as low as 10ng total RNA, purified mRNA or ribosomal-depleted RNA)
  • Fast workflow (5 – 5.5 hours), with minimal hands-on time
  • Robust, reliable performance
  • Ultra high fidelity amplification with minimized GC bias

Properties & Usage

Materials Required but not Supplied

  • NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) or NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310)
  • NEBNext Singleplex (NEB #E7350) or NEBNext Multiplex (NEB #E7335, #E7500 or #7600) Oligos for Illumina
  • Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
  • 80% Ethanol (freshly prepared)
  • Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
  • Agencourt RNAClean® XP (Beckman Coulter, Inc. #A63987) are required when using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310)
  • 0.1X TE, pH 8.0
  • 10mM Tris-HCl, pH 7.5–8.0
  • 10 mM NaCl (optional)


Companion Products
Citations
Additional Citations
  • Fustin, J.-M. et al. (2013) RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock Cell 155(4), 793-806.PubMedID: 24209618, DOI: 10.1016/j.cell.2013.10.026
Publications
  • Huang, L., Jin, J., Deighan, P., Liner, E., McReynolds, L. and Lieberman, J. (2013). Efficient and specific gene knockdown by small interfering RNAs produced in bacteria Nat Biotechnol. 31(4),PubMedID: 23475073, DOI: 10.1038/nbt.2537
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

This product is covered by one or more patents.

This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.

For additional information or to inquire about commercial use, please contact busdev@neb.com.

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