Anti-M13 pIII Monoclonal Antibody

Catalog # Concentration Size List Price Quantity Your Price
E8033S 0.10 ml
Please Inquire
Catalog # Size List Price Your Price
E8033S 0.10 ml
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Anti-M13 pIII Monoclonal Antibody (mouse isotype IgG2a) is derived from BALB/c mice immunized with the C-terminal half of M13 coat protein III (residues 259-406). Monoclonal anti-M13 pIII recognizes non-reduced and denatured reduced forms of wild type pIII or pIII fusions in immunoblotting and ELISA. ELISA assay against whole M13 phage is not recommended as the epitope lies in a region not accessible on intact M13 phage virions.

  • Can be used to determine fusion protein expression levels both in E.coli and on the M13 virion by immunoblot analysis
  • Recommended dilution for Western blot: 1:1000

Storage Temperature: -20°C

Anti-M13 pIII Monoclonal Antibody (mouse isotype IgG2a) is derived from BALB/c mice immunized with the C-terminal half of M13 coat protein III (residues 259-406). Monoclonal anti-M13 pIII recognizes nonreduced and denatured reduced forms of wild type pIII or pIII fusions in immunoblotting and ELISA. ELISA assay against whole M13 phage not recommended since the epitope lies in a region not accessible on intact M13 phage virions.
Application Features
  • Recombinant protein domains such as antibody fragments can be fused to M13 coat protein pIII and displayed on phage using phagemid-based systems (1), rather than the phage-based system utilized in the Ph.D. libraries. Anti-M13 pIII Monoclonal Antibody can be used to determine fusion protein expression levels both in E.coli and on the M13 virion by immunoblot analysis.

Properties & Usage

Antibody Type

Monoclonal

Isotype

Mouse Isotype IgG2a

Recommended Dilution for Western Blot

1:1000

Notes
  • Store undiluted
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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