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Ph.D.™-C7C Phage Display Peptide Library Kit

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E8120S 10 png expmts
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Catalog # Size List Price Your Price
E8120S 10 png expmts
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The Ph.D.™-C7C Phage Display Peptide Library Kit contains a tube of the Ph.D.-C7C Phage Library, protein reagents for a control reaction and sequencing primer stocks. The Ph.D.-C7C library is a combinatorial library of random peptides with a disulfide constrained loop, fused to a minor coat protein (pIII) of M13 phage. The displayed peptide, in the form AC-X7-CGGGS, is expressed at the N-terminus of pIII. The library consists of approximately 1 x 109 electroporated (i.e., unique) sequences.

  • Ready to use complex phage peptide library
  • Structurally constrained peptide library
  • Applications and methodologies are numerous and supported by over 20 years of literature citations
  • Does not require helper phage for amplification
  • Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube

The Ph.D.-C7C Phage Display Peptide Library is based on a combinatorial library of random peptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage. 


Please click the link for Applications of the Ph.D. Phage Display Peptide Libraries.


Complexity: 1.2 x 109 transformants.


Panning with a pentavalent peptide library displayed on plll.

Properties & Usage



Companion Products
Materials Sold Separately
References
  • Sidhu, S.S. et al. (2003). Chembiochem.. 4, 14-25.
  • Ferrer, M. et al. (1999). J. Pept. Res.. 54, 32-42.
  • BouHamdan, M. et al. (1998). J. Biol. Chem.. 273, 8009-8016.
  • Whaley, S.R. et al. (2000). Nature. 405, 665-668.
  • Rozinov, M.N. and Nolan, G.P. (1998). Chem. Biol.. 5, 713-728.
  • Rodi, D.J. et al. (1999). J. Mol. Biol.. 285, 197-203.
  • Kraft, S. et al. (1999). J. Biol. Chem.. 274, 1970-1985.
  • Koolpe, M. et al. (2002). J. Biol. Chem.. 277, 46974-46979.
  • Mummert, M.E. et al. (2000). J. Exp. Med.. 192, 769-779.
  • Hetian, L. et al. (2002). J. Biol. Chem.. 277, 43137-43142.
  • White, S.J. et al. (2001). Hypertension. 37, 449-455.
  • Azzazy, H.M. and Highsmith, W.E. (2002). Clin. Biochem.. 35, 425-445.
  • Binetruy-Tournaire, R. et al. (2000). EMBO J.. 19, 1525-1533.
  • Kragler, F. et al. (2000). EMBO J.. 19, 2856-2868.
  • Gazouli, M. et al. (2002). J. Pharmacol. Exp. Ther.. 303, 627-632.
  • Romanczuk, H. et al. (1999). Hum. Gene Ther.. 10, 2615-2626.
  • Nicklin, S.A. et al. (2000). Circulation. 102,
  • Jost, P.J. et al. (2001). FEBS Lett.. 489, 263-269.
  • Rasmussen, U.B. et al. (2002). Cancer Gene Ther.. 9, 606-612.
  • Tinoco, L.W. et al. (2002). J. Biol. Chem.. 277, 36351-36356.
  • Stratmann, J. et al. (2002). J. Clin. Microbiol.. 40, 4244-4250.
  • Mourez, M. et al. (2001). Nat. Biotechnol.. 19, 958-961.
  • Rodi, D.J. et al. (2002). Curr. Opin. Chem. Biol.. 6, 92-96.
  • Lee, L. et al. (2002). Arthritis Rheum.. 46, 2109-2120.
  • Duerr, D.M. et al. (2004). J. Virol. Methods. 116, 177-180.
  • Parmley, S.F. and Smith, G.P. (1988). Gene. 73, 305-318.
  • Berggard, T. et al. (2002). J. Biol. Chem.. 277, 41954-41959.
  • Chaudhary, J. et al. (2001). Am. J. Physiol. Cell Physiol.. 280, C1027-1030.
  • Chen, L. and Sigler, P.B. (1999). Cell. 99, 757-768.
  • Biorn, A.C. et al. (2004). Biochemistry. 43, 1928-1938.
  • Ferrer, M. and Harrison, S.C. (1999). J. Virol.. 73, 5795-5802.
Citations
Additional Citations
  • Matochko WL, Cory Li S, Tang SK, Derda R (2014) Prospective identification of parasitic sequences in phage display screens Nucleic Acids Res 42(3), 1784-98.PubMedID: 24217917, DOI: 10.1093/nar/gkt1104
  • Nguyen KT, Adamkiewicz MA, Hebert LE, Zygiel EM, Boyle HR, Martone CM, Meléndez-Ríos CB, Noren KA, Noren CJ, Hall MF (2014) Identification and characterization of mutant clones with enhanced propagation rates from phage-displayed peptide libraries Anal Biochem 462C, 35-43.PubMedID: 24952360, DOI: 10.1016/j.ab.2014.06.007
  • Kaur K, Taneja NK, Dhingra S, Tyagi JS (2014) DevR (DosR) mimetic peptides impair transcriptional regulation and survival of Mycobacterium tuberculosis under hypoxia by inhibiting the autokinase activity of DevS sensor kinase BMC Microbiol 14, 195.PubMedID: 25048654, DOI: 10.1186/1471-2180-14-195
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