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RNase If

Catalog # Concentration Size List Price Quantity Your Price
M0243L 50000 units/ml 25000 units $454.00
$408.60
M0243S 50000 units/ml 5000 units $115.00
$103.50
Catalog # Size List Price Your Price
M0243L 25000 units $454.00
$408.60
M0243S 5000 units $115.00
$103.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

RNase If eliminates single-stranded RNA from DNA and protein preparations 

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of DNA endonucleases and exonucleases 
  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.
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  • Monarch_MiniprepProtocol_VideoThumb
    Behind the Paper: E.coli RNase I exhibits a Ca2+-dependent double-stranded RNase activity 
Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5´ hydroxyl
and 2´,3´ cyclic monophosphate (1). It has a preference for single-stranded RNA over double-stranded RNA in the absence of calcium (2). RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.
Product Source
An E. coli strain containing a genetic fusion of the E. coli RNase I gene (2nd) and the gene coding for maltose binding protein (MBP).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  RNase If M0243SVIAL -20 1 x 0.1 ml 50,000 units/ml
  NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X
  RNase If M0243LVIAL -20 1 x 0.5 ml 50,000 units/ml
  NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X
Application Features
  • Degradation of single-stranded RNA to mono-, di- and trinucleotide (3)
  • Used in ribonuclease protection assays

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to fully digest 1 picomole of synthetic ssRNA 33-mer in a total reaction volume of 10 µl in 15 minutes in 1X NEBuffer 3 as visualized on a 20% acrylamide gel.

Reaction Conditions

1X NEBuffer™ 3
Incubate at 37°C

1X NEBuffer™ 3
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.5 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

70°C for 20 minutes

Materials Sold Separately
Notes
  • RNase If will not degrade DNA. It has a preference for single-stranded RNA over double-stranded RNA.
References
  • Spahr, P.F. and Hollingworth, B.R. (1961). J. Biol. Chem.. 236, 823-831.
  • Grünberg S, Coxam B, Chen TH, Dai N, Saleh L, Corrêa IR, Nichols NM, Yigit E. (2021). Nucleic Acids Res.. May 21;49(9), 5265-5277.
  • Meador, J. III and Kennell, D. (1990). Gene. 95, 1-7.
  • Meador, J. III, Cannon, B., Cannistraro, V.J. and Kennell, D. (1990). Eur. J. Biochem.. 187, 549-553.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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