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Micrococcal Nuclease

Catalog # Concentration Size List Price Quantity Your Price
M0247S 2000000 gel units/ml 320000 gel units $122.00
$109.80
Catalog # Size List Price Your Price
M0247S 320000 gel units $122.00
$109.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • DNA and RNA endonuclease
  • Degrades double-stranded and single-stranded DNA and RNA
  • Products are mononucleotides and oligonucleotides with terminal 3'-phosphates
  • Also known as Staphylococcal Nuclease
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Micrococcal Nuclease is ideal for:

  • Preparation of double-stranded DNA fragments with 5'-OH and 3'-phosphate
  • Studies of chromatin structure
  • Degradation of nucleic acids in crude cell-free extracts
  • Preparation of rabbit reticulocyte

 

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  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?
Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate (1). Both DNA and RNA are degraded to 3´ phosphomononucleotides and dinucleotides.

Micrococcal Nuclease works well with the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 from Cell Signaling Technology. Please follow the link to see product information and protocols on their website.

Figure 1:Figure 1
Digestion of 1 µg of Lambda genomic DNA with Micrococcal Nuclease in a 3-fold dilution series. The amount of enzyme used in Lane 2 is defined as 1 gel unit. Lane M is the PCR Marker (NEB #N3234).
Product Source
An E. coli strain containing a genetic fusion of the micrococcal nuclease gene (Gene ID: 3238436) and the gene coding for maltose binding protein, or MBP. The micrococcal nuclease is cleaved from the fusion protein and purified away from MBP.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Micrococcal Nuclease M0247SVIAL -20 1 x 0.16 ml 2,000,000 gel units/ml
  Micrococcal Nuclease Reaction Buffer B0247SVIAL -20 1 x 1.5 ml 10 X
  Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
Application Features
  • Degrade nucleic acids present in protein preparations
  • In vitro translation (2)
  • Reduce the viscosity of cell lysates during non-mechanic cell lysis preparation
  • Chromatic structure analysis (3)
  • Rapid RNA sequencing

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of Lambda DNA in 15 minutes at 37°C, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis.

Reaction Conditions

1X Micrococcal Nuclease Reaction Buffer
Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C

1X Micrococcal Nuclease Reaction Buffer
50 mM Tris-HCl
5 mM CaCl2
(pH 7.9 @ 25°C)

Storage Buffer

10 mM Tris-HCl
50 mM NaCl
1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

No

Companion Products
Materials Sold Separately
Notes
  • This enzyme does not work in NEBuffers r1.1-r3.1 or rCutSmart due to the lack of Ca2+. Additional Ca2+ in NEBuffers only shows 10% activity.
  • 1-5 mM Ca2+ is required for activity.
  • The enzyme is active in the pH range 7-10, with optimal activity at 9.2, as long as salt concentration is less than 100 mM.
  • Enzyme can be inactivated by addition of excess EGTA.
References
  • Cuatrecasas, S.F., and Anfinsen, C.B. (1967). J. Biol. Chem. 242, 1541-1547.
  • Craig, D. et al. (1992). Nucl. Acids Res. 20, 4957-4987.
  • O'Neill, L.P. and Turner B.M. (2003). Methods. 31, 76-82.
FAQs
Tech Tips
  • Addition of 1-5 mM Ca++ will increase activity if there is any EDTA or EGTA present
  • Addition of 1-5 mM EDTA or EGTA will chelate Ca++ and substantially decrease activity
  • Enzyme inhibited by higher than 100 mM NaCl
  • Used for chromatin structure analysis (CHiP)
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
Effective May 2, 2023, Recombinant Albumin (#B9200 at 20 mg/ml) replaced Purified BSA (#B9001 at 10 mg/ml).
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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