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Exonuclease V (RecBCD)

Catalog # Concentration Size List Price Quantity Your Price
M0345L 10000 units/ml 5000 units $494.00
$444.60
M0345S 10000 units/ml 1000 units $122.00
$109.80
Catalog # Size List Price Your Price
M0345L 5000 units $494.00
$444.60
M0345S 1000 units $122.00
$109.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • DNA specific exonuclease that also acts as an endonuclease on single-strand DNA
  • Initiates at both 5' and 3' termini of linear double-stranded DNA
  • Cleaves linear double-stranded DNA in both the 3' to 5' and 5' to 3' directions
  • Requires ATP in the reaction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease V is ideal for:

  • Degradation of contaminating linear DNA in plasmid samples
  • Removal of residual gDNA after purification of low copy plasmid
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3´ and 5´ ends) and processive, producing oligonucleotides (1,2,3). All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis (4,5).
Product Source
An E. coli strain containing plasmids for expressing the three subunits of E. coli Exonuclease V: RecB, RecC and RecD.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Exonuclease V (RecBCD) M0345SVIAL -20 1 x 0.1 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
  Adenosine 5'-Triphosphate (ATP) P0756SVIAL -20 1 x 1 ml 10 mM
  Exonuclease V (RecBCD) M0345LVIAL -20 1 x 0.5 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
  Adenosine 5'-Triphosphate (ATP) P0756SVIAL -20 1 x 1 ml 10 mM
Application Features
Degradation of linear ssDNA and dsDNA while preserving nicked and supercoiled plasmid DNA.

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in 30 minutes at 37°C in a total reaction volume of 50 μl.

Reaction Conditions

1X NEBuffer™ 4
Supplement with 1 mM ATP
Incubate at 37°C

1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

50 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.5 @ 25°C

Heat Inactivation

70°C for 30 minutes

Unit Assay Conditions

1X NEBuffer 4, 1 mM ATP with 0.15 mM sonicated duplex [3H]-DNA.

Companion Products
Materials Sold Separately
References
  • Eichler, D.C. et al. (1977). J.Biol. Chem. 252, 499-503.
  • Taylor, A.F. et al. (1985). J. Mol. Biol. 185, 431-443.
  • Amundsen, S.K. et al. (1986). Proc. Natl. Acad. Sci. 83, 5558-5562.
  • Palas, K.M. et al. (1990). J. Biol. Chem. 265, 3447-3454.
  • Dillingham, M.S. et al. (2008). Microbiology and Mol. Biol. Review. 72, 642-671.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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