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OneTaq® Hot Start 2X Master Mix with Standard Buffer

Catalog # Concentration Size List Price Quantity Your Price
M0484L 2 X 500 reactions $527.00
$474.30
$316.20
M0484S 2 X 100 reactions $117.00
$105.30
$70.20
Catalog # Size List Price Your Price
M0484L 500 reactions $527.00
$474.30
$316.20
M0484S 100 reactions $117.00
$105.30
$70.20
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

  • Obtain high yields across a wide range of AT / GC content
  • 2X higher fidelity than Taq
  • Hot Start feature enables room temperature reaction setup
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification
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    Overview of PCR

OneTaq® Hot Start 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgCl2 and other buffer components and stabilizers listed below, requiring only the addition of primers and DNA template for robust amplification

Comparison of OneTaq Products to Other Commercially Available Polymerases

OneTaq_ComparisonChart_0220 
Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.


Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  OneTaq® Hot Start 2X Master Mix with Standard Buffer M0484SVIAL -20 2 x 1.25 ml 2 X
  OneTaq® Hot Start 2X Master Mix with Standard Buffer M0484SVIAL -20 10 x 1.25 ml 2 X
Application Features
  • High Throughput PCR
  • High Sensitivity PCR
  • Routine PCR
  • AT-rich PCR
  • Colony PCR
  • Long PCR (up to ~6 kb genomic)

Properties & Usage

1X Master Mix Composition

20 mM Tris-HCl
22 mM KCl
22 mM NH4Cl
1.8 mM MgCl2
5% Glycerol
0.06% IGEPAL® CA-630
0.05% Tween® 20
0.2 mM dNTPs
25 units/ml OneTaq Hot Start DNA Polymerase
pH 8.9 @ 25°C

Heat Inactivation

No

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. 

Companion Products
Notes
  • Product specifications for individual components in the OneTaq Hot Start 2X Master Mix with Standard Buffer are available separately.
  • OneTaq Hot Start 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
  • OneTaq Hot Start 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.
References
  • Barnes, W.M (1994). Proc.Natl.Acad.Sci.USA. 91, 2216-2220.
  • Saiki, R.K. et al. (1994). Science. 91, 2216-2220.
  • Powell, L.M. et al. (1987). Cell. 50, 831-840.
  • Sun, Y, Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
Citations
Additional Citations
  • Gorski L, Walker S, Liang AS, Nguyen KM, Govoni J, et al. (2014) Comparison of Subtypes of Listeria monocytogenes Isolates from Naturally Contaminated Watershed Samples with and without a Selective Secondary Enrichment PLoS One 9(3), e92467.PubMedID: 24651315, DOI: 10.1371/journal.pone.0092467
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the OneTaq® Hot Start 2X Master Mix with Standard Buffer for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.

 NEW ENGLAND BIOLABS®, ONETAQ®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc. 
DEEPVENT® is a trademark of New England Biolabs, Inc. 

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas LLC. 

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