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Histone H2A/H2B Dimer Human, Recombinant

Catalog # Concentration Size List Price Quantity Your Price
M2508S 20 µM 2 nmol
Please Inquire
Catalog # Size List Price Your Price
M2508S 2 nmol
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Histone H2A combines with Histone H2B to form the H2A/H2B heterodimer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer.
  • Histones are modified by various enzymes and can act as substrates for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the dimer may be a better substrate for specific enzymes and modifications.
  • Highly pure, recombinant protein
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination

 

Protein Sequence: Human Histone H2A Protein Sequence: SGRGK QGGKA RAKAK SRSSR AGLQF PVGRV HRLLR KGNYS ERVGA GAPVY LAAVL EYLTA EILEL AGNAA RDNKK TRIIP RHLQL AIRND EELNK LLGRV TIAQG GVLPN IQAVL LPKKT ESHHK AKGK (Genbank accession number: AAN59960)

Human Histone H2B Protein Sequence: PEPAK SAPAP KKGSK KAVTK AQKKD GKKRK RSRKE SYSIY VYKVL KQVHP DTGIS SKAMG IMNSF VNDIF ERIAG EASRL AHYNK RSTIT SREIQ TAVRL LLPGE LAKHA VSEGT KAVTK YTSSK(Genbank accession number: AAN59961)

SDS-PAGE analysis of Histone H2A/H2B Dimer, Recombinant
SDS-PAGE analysis of Histone H2A/H2B Dimer, Recombinant
Lane 1&7: NEB Protein Ladder (NEB #P7703)
Lane 2: Histone H2A (NEB #M2502S)
Lane 3: Histone H2B (NEB #M2505S)
Lane 4–6: 2.0, 4.0, 8.0 µg Histone H2A/H2B Dimer
Product Source
Purified H2A and H2B (NEB #M2502 and NEB #M2505) were denatured, refolded and the dimer was purified by gel filtration.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Histone H2A/H2B Dimer Human, Recombinant M2508SVIAL -20 1 x 0.1 ml 20 µM

Properties & Usage

Storage Buffer

20 mM Tris-HCl
2 M NaCl
1 mM DTT
1 mM EDTA
pH 8 @ 25°C

Molecular Weight

Theoretical: 27779 daltons


Companion Products
Notes
  • 20 μM, 0.55 mg/ml is calculated using the molar extinction coefficient for Histone H2A/H2B Dimer (11,920) and its absorbance at 280 nm (4, 5)
  • To use as a substrate in an enzyme modification assay or other salt sensitive protocol, use 1 to 2 µl of the dimer in a minimum 20 µl reaction so that the salt concentration in the reaction ≤ 200 mM.
  • Because of high salt, the solution does not always remain frozen.
References
  • Kornberg, R.D. (1977). Annu. Rev. Biochem. 46, 931-954.
  • van Holde, K.E. (1989). Chromatin. 1-497,
  • Mersha, F. unpublished observations.
  • Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. 182, 319-326.
  • Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.
Tech Tips
  • To use as a substrate in an enzyme modification assay or other salt sensitive protocol, use 1 to 2 μl of the dimer in a minimum 20 μl reaction so that the salt concentration in the reaction = 200 mM.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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