Product Class: Other

Low Range ssRNA Ladder
Item# Description List Price Web Price Qty
N0364S Low Range ssRNA Ladder - 25 gel lanes $98.00
$88.20
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
X

Companion Products

Item# Description List Price Web Price Qty
N2102S microRNA Marker - 100 gel lanes $98.00
$88.20
ADD TO CART

Product Introduction

  • Size range: 50 bases - 1,000 bases
  • Compatible with denaturing polyacrylamide-urea gels, denaturing agarose gels and native agarose gels
  • Convenient reference band at 300 bases
Bioz Badge Exists : True

Product Information

Description

The Low Range ssRNA Ladder is a set of 6 RNA molecules produced by in vitro transcription of a mixture of 6 linear DNA templates. The ladder sizes are: 1000, 500, 300, 150, 80 and 50 bases. The 300 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing polyacrylamide-urea gels, and on denaturing or native agarose gels.

Usage Recommendation
This marker was not designed for precise quantification of ssRNA mass.

Denaturing vs. Native Agarose Gels
It is common practice to electrophorese RNA on a fully denaturing agarose gel, such as one containing formaldehyde (1). However, in many cases it is possible to run RNA on a native agarose gel and obtain suitable results. In fact, it has been demonstrated that treatment of RNA samples in a denaturing buffer maintains the RNA molecules in a denatured state, during electrophoresis, for at least 3 hours (2,3). The use of native agarose gels eliminates problems associated with toxic chemicals and the difficulties encountered when staining and blotting formaldehyde gels.

Sample Preparation
The Low Range ssRNA Ladder is also compatible with formaldehydebased loading buffers.

Properties & Usage

    Storage Temperature

    -20°C

    Storage Conditions

    20 mM sodium citrate
    1 mM EDTA
    pH 6.0 @ 25°C

    Product Notes

    1. Minimize repeated freeze-thawcycles. It is best to aliquot the marker into singleuse portions.

      To avoid ribonuclease contamination: wear gloves,use RNase-free water for gels and buffers, washequipment with detergent and rinse thoroughlywith RNase-free water.

    2. Store at -20˚C for long-term storage. For short-term storage (<1 week), store at 4˚C.

    References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 2nd ed., 743-745.
    2. Liu, Y-C. and Chou, Y-C. (1990). Biotechniques. 9,
    3. Sandra Cook, and Christina Marchetti Unpublished observation
    4. Dong Ma, and John Greci Unpublished observation

    Protocols, Manuals & Usage

    Protocols

    1. Sample preparation using provided buffer (N0364)

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

    FAQs & Troubleshooting

    FAQs

    1. I need to know what types of gel to use to run RNA ladders?
    2. What types of gel and staining dyes can I use for NEB’s Low Range ssRNA Ladder (N0364S)?
    3. Why the bands of the Low Range ssRNA Ladder on my 2% agarose-TBE gel are not resolved well?
    4. Why do I have extra bands of the Low Range ssRNA Ladder on my denaturing 6% TBE-Urea gel?
    5. Will the Low Range ssRNA Ladder run on 10% TBE-Urea and 15% TBE-Urea gels?

    Troubleshooting

    Tech Tips

    For running a denaturing polyacrylamide gel such as 6% TBE-Urea gel, one needs to load only 0.03µg-0.0625µg of this ladder per lane. For running a non-denaturing 2% agarose-TBE gel, one needs to load 0.25µg-0.5µg of this ladder per lane. 

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

    Other Products You May Be Interested In