Product Class: Other

λ DNA-BstEII Digest
Item# Description List Price Web Price Qty
N3014S Lambda DNA-BstE II Digest - 150 gel lanes $100.00
$90.00
ADD TO CART
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

 

Product Introduction

  • Size range: 117 bp to 8,454 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
Bioz Badge Exists : True

Product Information

Description

The BstEII digest of lambda DNA (cI857 ind 1 Sam 7) yields 14 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

  • Recommended gel percentage range: 0.6-1.2%
  • Optimum separation on 0.8% 

Properties & Usage

    Bases

    Fragment Mass bp
    1 174 8,454
    2 149 7,242
    3 131 6,369
    4 117 5,686
    5 99 4,822
    6 89 4,324
    7 76 3,675
    8 48 2,323
    9 40 1,929
    10 28 1,371
    11 26 1,264
    12 14 702
    13 5 224
    14 2 117

    Effective Size Range

    117.0bp to 8,454.0bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Product Notes

    1. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20 and subsequently heated. Temperatures > 60°C may cause denaturation.
    2. The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
    3. 1X Gel Loading Dye, Purple, no SDS: 
      2.5% Ficoll®-400
      10mM EDTA
      3.3mM Tris-HCl (pH [email protected]°C)
      0.02% Dye 1
      0.001% Dye 2

    References

    1. Daniels, D.L. et al (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
    2. Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.

    Protocols, Manuals & Usage

    Protocols

    1. Suggested Loading Protocol for N3014 & N3012

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

    FAQs & Troubleshooting

    FAQs

    1. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Why does my Lambda DNA molecular weight marker appear smeared and not represent the correct banding pattern?
    4. Can I use Midori Green with the DNA Ladders from NEB?
    5. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
    6. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

    Troubleshooting

    Tech Tips

    Heat at 60C for 3 minutes to separate the cohesive ends of fragments 1 and 4.

    To make it ready-to-load, dilute in TE buffer instead of water.

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.