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EcoRI

Catalog # Concentration Size List Price Quantity Your Price
R0101L 20000 units/ml 50000 units $409.00
$368.10
R0101M 100000 units/ml 50000 units $409.00
$368.10
R0101S 20000 units/ml 10000 units $100.00
$90.00
R0101T 100000 units/ml 10000 units $100.00
$90.00
Catalog # Size List Price Your Price
R0101L 50000 units $409.00
$368.10
R0101M 50000 units $409.00
$368.10
R0101S 10000 units $100.00
$90.00
R0101T 10000 units $100.00
$90.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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    Double Digestion with NEBcloner
EcoRI has a High Fidelity version EcoRI-HF® (NEB #R3101).

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.
Product Source
An E. coli strain that carries the cloned EcoRI gene from E. coli RY13 (R.N. Yoshimori).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  EcoRI R0101SVIAL -20 1 x 0.5 ml 20,000 units/ml
  NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
  EcoRI R0101TVIAL -20 1 x 0.1 ml 100,000 units/ml
  NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
  EcoRI R0101LVIAL -20 2 x 1.25 ml 20,000 units/ml
  NEBuffer™ EcoRI/SspI B0101SVIAL -20 2 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
  EcoRI R0101MVIAL -20 1 x 0.5 ml 100,000 units/ml
  NEBuffer™ EcoRI/SspI B0101SVIAL -20 1 x 1.25 ml 10 X
  Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ EcoRI/SspI
Incubate at 37°C

1X NEBuffer™ EcoRI/SspI
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl2
0.025% Triton® X-100
(pH 7.5 @ 25°C)

Activity in NEBuffers

NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 50%
rCutSmart™ Buffer: 50%

Diluent Compatibility

Storage Buffer

10 mM KPO4
300 mM NaCl
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
1 mM DTT
0.15% Triton® X-100
pH 7 @ 25°C

Heat Inactivation

65°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping

Isoschizomers

EcoRI-HF



Companion Products
Materials Sold Separately
Notes
  • May exhibit star activity in NEBuffer r2.1 or rCutSmart Buffer.
  • This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol..
  • Blocked by some combinations of overlapping CpG methylation.
Citations
Additional Citations
  • Tong C, Li H, Wang Y, Li X, Ou J, Wang D, Xu H, Ma C, Lang X, Liu G, Zhang B, Shi J. (2016) Construction of High-Density Linkage Maps of Populus deltoides × P. simonii Using Restriction-Site Associated DNA Sequencing. PLoS One Mar 10;11(3), e0150692.
  • Yang H, Wei CL, Liu HW, Wu JL, Li ZG, Zhang L, Jian JB, Li YY, Tai YL, Zhang J, Zhang ZZ, Jiang CJ, Xia T, Wan XC. (2016) Genetic Divergence between Camellia sinensis and Its Wild Relatives Revealed via Genome-Wide SNPs from RAD Sequencing. PLoS One Mar 10;11(3), e0151424.
  • Mousavi M, Tong C, Liu F, Tao S, Wu J, Li H, Shi J. (2016) De novo SNP discovery and genetic linkage mapping in poplar using restriction site associated DNA and whole-genome sequencing technologies. BMC Genomics Aug 18;17, 656.
  • Xiao B, Tan Y, Long N, Chen X, Tong Z, Dong Y, Li Y. (2015) SNP-based genetic linkage map of tobacco (Nicotiana tabacum L.) using next-generation RAD sequencing. J Biol Res (Thessalon) Oct 6;22:11. ,
  • Zhang Q, Liu C, Liu Y, VanBuren R, Yao X, Zhong C, Huang H. (2015) High-density interspecific genetic maps of kiwifruit and the identification of sex-specific markers. DNA Res Oct;22(5), 367-75.
  • Bonatelli IA, Carstens BC, Moraes EM. (2015) Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae). PLoS One Nov 11;10(11), e0142602.
  • Fischer G, Azorsa F, Garcia FH, Mikheyev AS, Economo EP. (2015) Two new phragmotic ant species from Africa: morphology and next-generation sequencing solve a caste association problem in the genus Carebara Westwood. Zookeys Oct 5;(525), 77-105.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications

EcoRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227213. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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