NEB® 5-alpha F'Iq Competent E. coli (High Efficiency)

Catalog # Concentration Size List Price Quantity Your Price
C2992H 20 x 0.05 ml $326.00
$293.40
C2992I 6 x 0.2 ml $255.00
$229.50
Catalog # Size List Price Your Price
C2992H 20 x 0.05 ml $326.00
$293.40
C2992I 6 x 0.2 ml $255.00
$229.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEB 5-alpha F'Iq Competent E. coli is a derivative of the popular DH5α.

  • Extremely high transformation efficiency suitable for toxic gene cloning
  • Tight expression control (lacIq)
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
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Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.
Highlights
  • DH5α™ derivative
  • Free of animal products
  • Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
  • Tight control of expression by laclq allows potentially toxic genes to be cloned
  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • Resistance to phage T1 (fhuA2)
  • Suitable for blue/white screening by α-complementation of the β-galactosidase gene
  • F' allows cells to be infected with bacteriophage M13 for ssDNA production
  • Reduced recombination of cloned DNA (recA1)
  • K12 Strain
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  NEB® 5-alpha F'Iq Competent E. coli (High Efficiency) C2992HVIAL -80 20 x 0.05 ml
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  NEB® 5-alpha F'Iq Competent E. coli (High Efficiency) C2992IVIAL -80 6 x 0.2 ml
Features
  • Toxic gene cloning
  • F´ strain with extremely high transformation efficiency
Application Features

DNA Effects on Transformation Efficiency and Colony Output:

DNA Effects on Transformation Efficiency and Colony Output

The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng.




Effect of heat shock time on NEB 5-alpha F´Iq competent E.coli transformation efficiency: 

Effect of heat shock time on NEB 5-alpha F´Iq competent E.coli transformation efficiency:

50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.




Effect of DNA incubation time on NEB 5-alpha F´Iq competent E.coli transformation efficiency: 

Effect of DNA incubation time on NEB 5-alpha F´Iq competent E.coli transformation efficiency:

50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.




Effect of outgrowth medium on transformation efficiency: 

Effect of outgrowth medium on transformation efficiency:

50 μl of NEB 5-alpha F´Iq competent E.coli was transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol with the exception of varying the outgrowth medium. NEB SOC outgrowth medium delivers the highest transformation efficiency.




Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection Working Concentration
Ampicillin 100 µg/ml
Carbenicillin 100 µg/ml
Chloramphenicol 33 µg/ml
Kanamycin 30 µg/ml
Streptomycin 25 µg/ml

Shipping Notes

  • Ships on dry ice

Antibiotic Resistance

  • tetracycline


Materials Sold Separately
Notes
  • CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
  • STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
Additional Citations
  • Firnberg E, Ostermeier M (2013) The genetic code constrains yet facilitates Darwinian evolution Nucleic Acids Res 41(15), 7420-8.PubMedID: 23754851, DOI: 10.1093/nar/gkt536
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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