Quick Blunting™ and Quick Ligation™ Kits

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E0542S		20 reactions	$264.00		$237.60

Catalog #	Size	List Price	Your Price
E0542S	20 reactions	$264.00	$237.60

NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.

The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.

The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E0542S -20

Quick Ligase	M2200SVIAL	-20	1 x 0.03 ml
Quick Ligation Reaction Buffer	B2200SVIAL	-20	1 x 1 ml	2 X
Blunting Enzyme Mix	M1201AVIAL	-20	1 x 0.025 ml
10X Blunting Buffer	B1201SVIAL	-20	1 x 0.5 ml	10 X
Deoxynucleotide Solution Mix (1 mM)	N1201AVIAL	-20	1 x 0.1 ml	1 mM

Properties & Usage

Companion Products

Notes

Please note storage conditions for individual products.

Protocols

Usage Guidelines

Tips for Maximizing Ligation Efficiencies

Web Tools

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

E0542S_v0_10009145
E0542S_v0_10021288
E0542L_v0_10023229
E0542S_v0_10037246
E0542L_v0_10040085
E0542L_v0_10042834
E0542S_v0_10045944
E0542S_v0_10049717
E0542L_v0_10052507
E0542S_v0_10057688
E0542S_v0_10058707
E0542S_v0_10066882
E0542L_v0_10065803
E0542S_v0_10070023
E0542L_v0_10070595
E0542S_v0_10093417
E0542S_v0_10104026
E0542S_v0_10112467
E0542S_v0_10122066
E0542S_v0_10138058
E0542S_v0_10145591
E0542S_v0_10152899
E0542S_v0_10157413
E0542S_v0_10162755
E0542S_v0_10167430
E0542S_v0_10176798
E0542S_v0_10187344
E0542S_v0_10197836
E0542S_v0_10206261
E0542S_v0_10228841

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.