The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent® includes enzymatic DNA fragmentation in a streamlined, high-performance library prep workflow.
The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent® includes a master mix of reagents for enzymatic DNA fragmentation combined with end repair. This enables efficient and tunable DNA shearing and blunting with minimized sample loss before performing adaptor ligation and high-fidelity library amplification. The kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time.
Adaptors and primers for singleplex libraries are supplied in the kits. For multiplexed libraries, the Ion Xpress™ Barcode Adapters from Thermo Fisher Scientific can be used.The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E6285L | -20 |
NEBNext DNA Library Primers for Ion Torrent™ | E6291AAVIAL | -20 | 1 x 0.5 ml | ||
T4 DNA Ligase | E6288AAVIAL | -20 | 1 x 0.2 ml | ||
Magnesium Chloride (MgCl2) (200 mM) | E6295AAVIAL | -20 | 1 x 0.25 ml | ||
NEBNext Stop Buffer | E6294AAVIAL | -20 | 1 x 0.25 ml | ||
NEBNext DNA Fragmentation Reaction Buffer | E6287AAVIAL | -20 | 1 x 0.1 ml | ||
NEBNext DNA Library Adaptors for Ion Torrent | E6290AAVIAL | -20 | 1 x 0.5 ml | ||
NEBNext DNA Fragmentation Master Mix | E6286AAVIAL | -20 | 1 x 0.1 ml | ||
T4 DNA Ligase Buffer for Ion Torrent | E6296AAVIAL | -20 | 1 x 0.2 ml | ||
TE Buffer | E6299AAVIAL | -20 | 1 x 7.75 ml | 0.1 X | |
Bst 2.0 WarmStart® DNA Polymerase | E6277AAVIAL | -20 | 1 x 0.05 ml | ||
NEBNext Q5 Hot Start HiFi PCR Master Mix | E6629AAVIAL | -20 | 2 x 1.25 ml | 2 X |
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™ for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.
NEBNEXT®, NEW ENGLAND BIOLABS®, WARMSTART® and Q5® are registered trademarks of New England Biolabs, Inc.