NEBNext® Ultra™ Ligation Module

Catalog # Concentration Size List Price Quantity Your Price
E7445L 96 reactions $2,027.00
$1,824.30
E7445S 24 reactions
Please Inquire
Catalog # Size List Price Your Price
E7445L 96 reactions $2,027.00
$1,824.30
E7445S 24 reactions
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
This module is part of the original Ultra™ workflow, and is optimized for use with the NEBNext® Ultra End Repair/dA-Tailing Module (NEB #E7445), for Illumina®-compatible library construction. The original Ultra workflow enables library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol. However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 µg), and a NEBNext Ultra II Ligation Module (NEB #E7595) is available.




 
The NEBNext® Ultra™ Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7445), and is part of the original Ultra workflow, which achieves library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol. 

The NEBNext Ultra™ Ligation Module is Designed for Use with the Following Products:
NEBNext Ultra™ End Repair/dA-Tailing Module (NEB #E7442)
NEBNext Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335#E7500, #E7710, #E7730, #E6609, #E7600)
NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543)
NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® Ligation Enhancer E7374AAVIAL -20 1 x 0.096 ml
  Blunt/TA Ligase Master Mix E7373AAVIAL -20 2 x 0.72 ml

Properties & Usage

Materials Required but not Supplied

  • NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7442)
  • NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500 or #E7600)
  • NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543)
  • NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)
  • Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)


Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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