Product Class:

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2)
Item# Description List Price Web Price Qty
E7500L NEBNext Multiplex Oligos for Illumina (Index Primers Set 2) - 96 rxns $547.00
$492.30
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E7500S NEBNext Multiplex Oligos for Illumina (Index Primers Set 2) - 24 rxns $149.00
$134.10
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
E6040L NEBNext DNA Library Prep Master Mix Set for Illumina - 60 rxns $2,028.00
$1,825.20
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E6040S NEBNext DNA Library Prep Master Mix Set for Illumina - 12 rxns $507.00
$456.30
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E6056L NEBNext Quick Ligation Module - 100 rxns $1,738.00
$1,564.20
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E6056S NEBNext Quick Ligation Module - 20 rxns $435.00
$391.50
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E6240L NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina - 60 rxns $1,652.00
$1,486.80
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E6240S NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina - 12 rxns $414.00
$372.60
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E7370L NEBNext Ultra DNA Library Prep Kit for Illumina - 96 rxns $2,948.00
$2,653.20
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E7370S NEBNext Ultra DNA Library Prep Kit for Illumina - 24 rxns $771.00
$693.90
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E7420L NEBNext Ultra Directional RNA Library Prep Kit for Illumina - 96 rxns $5,398.00
$4,858.20
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E7420S NEBNext Ultra Directional RNA Library Prep Kit for Illumina - 24 rxns $1,664.00
$1,497.60
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E7445L NEBNext Ultra Ligation Module - 96 rxns $1,664.00
$1,497.60
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E7445S NEBNext Ultra Ligation Module - 24 rxns $518.00
$466.20
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E7530L NEBNext Ultra RNA Library Prep Kit for Illumina - 96 rxns $4,864.00
$4,377.60
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E7530S NEBNext Ultra RNA Library Prep Kit for Illumina - 24 rxns $1,521.00
$1,368.90
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E7595L NEBNext Ultra II Ligation Module - 96 rxns $1,803.00
$1,622.70
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E7595S NEBNext Ultra II Ligation Module - 24 rxns $561.00
$504.90
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E7645L NEBNext Ultra II DNA Library Prep Kit for Illumina - 96 rxns $3,188.00
$2,869.20
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E7645S NEBNext Ultra II DNA Library Prep Kit for Illumina - 24 rxns $838.00
$754.20
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    Product Introduction

    NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

    Additional NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.


    •  High efficiency adaptor ligation
    •  Minimized adaptor-dimer formation
    •  Includes index primers for library multiplexing

    Product Information

    Description



    Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

    NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

    Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7710, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

    For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.


    Figure 1: Workflow
    Figure 1: Workflow


    Functional Validation

    Each set of reagents is functionally validated together through construction and sequencing of genomic DNA libraries on the Illumina sequencing platform.

    Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBNext Universal PCR Primer for Illumina -20
    NEBNext Index 13 Primer for Illumina -20
    NEBNext Index 14 Primer for Illumina -20
    NEBNext Index 15 Primer for Illumina -20
    NEBNext Index 18 Primer for Illumina -20
    NEBNext Index 19 Primer for Illumina -20
    NEBNext Index 20 Primer for Illumina -20
    NEBNext Index 21 Primer for Illumina -20
    NEBNext Index 22 Primer for Illumina -20
    NEBNext Index 23 Primer for Illumina -20
    NEBNext Index 25 Primer for Illumina -20
    NEBNext Index 27 Primer for Illumina -20
    NEBNext Adaptor for Illumina -20
    USER® Enzyme -20
    NEBNext® Index 16 Primer for Illumina®
    Product Categories:
    Adaptors and Primers for Library Prep Products,
    ChIP-Seq Library Prep Products,
    FFPE DNA Products
    ,
    Microbiome DNA Enrichment Products
    ,
    DNA Library Prep Products
    ,
    RNA Library Prep Products
    Applications:
    DNA for Illumina,
    Illumina Library Preparation,
    Applications of USER® and Thermolabile USER II Enzymes

    Properties & Usage

    Materials Required but not Supplied

    For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.

    Storage Temperature

    -20°C

    Protocols, Manuals & Usage

    Protocols

    1. Refer to the manual for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

    Faqs & Troubleshooting

    FAQs

    1. Are NEBNext adaptors and primers for Illumina validated in Next Generation Sequencing Workflows?
    2. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
    3. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
    4. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
    5. Are these reagents available in a customized format, larger format or 96 well format?
    6. Can USER Enzyme be purchased separately?
    7. Are the NEBNext Oligos for Illumina compatible with single read and paired end sequencing?
    8. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
    9. How do I know the concentration of my index primers? 
    10. What should I do if the sample sheet from the NEB.com website is not compatible with the software version I am using?

    Troubleshooting

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.