Product Class:

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)
Item# Description List Price Web Price Qty
E7600S NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) - 96 rxns $666.00
$599.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
E6040L NEBNext DNA Library Prep Master Mix Set for Illumina - 60 rxns $2,069.00
$1,862.10
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E6040S NEBNext DNA Library Prep Master Mix Set for Illumina - 12 rxns $517.00
$465.30
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E6056L NEBNext Quick Ligation Module - 100 rxns $1,773.00
$1,595.70
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E6056S NEBNext Quick Ligation Module - 20 rxns $444.00
$399.60
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E6177L NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads - 96 rxns $3,803.00
$3,422.70
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E6177S NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads - 24 rxns $1,007.00
$906.30
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E6240L NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina - 60 rxns $1,685.00
$1,516.50
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E6240S NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina - 12 rxns $422.00
$379.80
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E7103L NEBNext Ultra II DNA Library Prep with Sample Purification Beads - 96 rxns $3,570.00
$3,213.00
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E7103S NEBNext Ultra II DNA Library Prep with Sample Purification Beads - 24 rxns $934.00
$840.60
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E7370L NEBNext Ultra DNA Library Prep Kit for Illumina - 96 rxns $3,007.00
$2,706.30
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E7370S NEBNext Ultra DNA Library Prep Kit for Illumina - 24 rxns $786.00
$707.40
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E7420L NEBNext Ultra Directional RNA Library Prep Kit for Illumina - 96 rxns $5,506.00
$4,955.40
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E7420S NEBNext Ultra Directional RNA Library Prep Kit for Illumina - 24 rxns $1,697.00
$1,527.30
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E7445L NEBNext Ultra Ligation Module - 96 rxns $1,697.00
$1,527.30
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E7445S NEBNext Ultra Ligation Module - 24 rxns $528.00
$475.20
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E7530L NEBNext Ultra RNA Library Prep Kit for Illumina - 96 rxns $4,961.00
$4,464.90
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E7530S NEBNext Ultra RNA Library Prep Kit for Illumina - 24 rxns $1,551.00
$1,395.90
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E7595L NEBNext Ultra II Ligation Module - 96 rxns $1,839.00
$1,655.10
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E7595S NEBNext Ultra II Ligation Module - 24 rxns $572.00
$514.80
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E7645L NEBNext Ultra II DNA Library Prep Kit for Illumina - 96 rxns $3,252.00
$2,926.80
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E7645S NEBNext Ultra II DNA Library Prep Kit for Illumina - 24 rxns $855.00
$769.50
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E7760L NEBNext Ultra II Directional RNA Library Prep Kit for Illumina - 96 rxns $4,696.00
$4,226.40
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E7760S NEBNext Ultra II Directional RNA Library Prep Kit for Illumina - 24 rxns $1,383.00
$1,244.70
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E7765L NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads - 96 rxns $5,213.00
$4,691.70
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E7765S NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads - 24 rxns $1,531.00
$1,377.90
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E7770L NEBNext Ultra II RNA Library Prep Kit for Illumina - 96 rxns $4,474.00
$4,026.60
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E7770S NEBNext Ultra II RNA Library Prep Kit for Illumina - 24 rxns $1,317.00
$1,185.30
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E7775L NEBNext Ultra II RNA Library Prep with Sample Purification Beads - 96 rxns $4,955.00
$4,459.50
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E7775S NEBNext Ultra II RNA Library Prep with Sample Purification Beads - 24 rxns $1,457.00
$1,311.30
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E7805L NEBNext Ultra II FS DNA Library Prep Kit for Illumina - 96 rxns $3,476.00
$3,128.40
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E7805S NEBNext Ultra II FS DNA Library Prep Kit for Illumina - 24 rxns $920.00
$828.00
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Product Introduction

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.

Additional NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Increased sample identification specificity

Product Information

Description




Incorporating a novel hairpin loop structure, the NEBNext® Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

Dual Index Workflow
Dual Index Workflow

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
NEBNext Adaptor for Illumina -20
USER® Enzyme -20
NEBNext i501 Primer -20
NEBNext i502 Primer -20
NEBNext i503 Primer -20
NEBNext i504 Primer -20
NEBNext i505 Primer -20
NEBNext i506 Primer -20
NEBNext i507 Primer -20
NEBNext i508 Primer -20
NEBNext i701 Primer -20
NEBNext i702 Primer -20
NEBNext i703 Primer -20
NEBNext i704 Primer -20
NEBNext i705 Primer -20
NEBNext i706 Primer -20
NEBNext i707 Primer -20
NEBNext i708 Primer -20
NEBNext i709 Primer -20
NEBNext i710 Primer -20
NEBNext i711 Primer -20
NEBNext i712 Primer -20
Product Categories:
Adaptors and Primers for Library Prep Products,
ChIP-Seq Library Prep Products,
FFPE DNA Products
,
Microbiome DNA Enrichment Products
,
DNA Library Prep Products
,
RNA Library Prep Products
Applications:
DNA for Illumina,
Illumina Library Preparation,
Applications of USER® and Thermolabile USER II Enzymes

Properties & Usage

Materials Required but not Supplied

For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.

Storage Temperature

-20°C

Protocols, Manuals & Usage

Protocols

  1. Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (E7600)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

  • Sample Sheet MiSeq E7600
  • Sample Sheet NextSeq E7600

Faqs & Troubleshooting

FAQs

  1. Do you have Sample Sheets for use with the NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)?
  2. Do NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)come with library prep reagents?
  3. Can I use NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) for library prep from both DNA and RNA samples?
  4. How many libraries can I prepare with the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)?
  5. For pooling <96 samples, what combination of indices should I use?
  6. Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) on the Illumina platforms?
  7. Can I perform single read runs and still get both index sequences? 
  8. Can I run both single read and paired-end recipes with dual-indexed libraries? 
  9. How does dual indexing work? 
  10. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
  11. What should I do if the sample sheet from the NEB.com website is not compatible with the software version I am using?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.