NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
E7730L		96 reactions	$608.00		$547.20
E7730S		24 reactions	$165.00		$148.50

Catalog #	Size	List Price	Your Price
E7730L	96 reactions	$608.00	$547.20
E7730S	24 reactions	$165.00	$148.50

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER^®Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

E7730S -20

USER^® Enzyme	E7338AVIAL	-20	1 x 0.072 ml
NEBNext Adaptor for Illumina	E7337AVIAL	-20	1 x 0.24 ml
NEBNext Universal PCR Primer for Illumina	E6861AVIAL	-20	1 x 0.12 ml	10 µM
NEBNext Index 37 Primer for Illumina	E7731AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 38 Primer for Illumina	E7732AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 39 Primer for Illumina	E7733AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 41 Primer for Illumina	E7735AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 43 Primer for Illumina	E7737AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 44 Primer for Illumina	E7738AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 45 Primer for Illumina	E7739AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 46 Primer for Illumina	E7740AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 47 Primer for Illumina	E7741AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 48 Primer for Illumina	E7742AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext® Index 40 Primer for Illumina®	E7744AVIAL	-20	1 x 0.01 ml	10 µM
NEBNext Index 30 Primer for Illumina	E7716AVIAL	-20	1 x 0.01 ml	10 µM

E7730L -20

USER^® Enzyme	E7338AAVIAL	-20	1 x 0.288 ml
NEBNext Adaptor for Illumina	E7337AAVIAL	-20	1 x 0.96 ml
NEBNext Universal PCR Primer for Illumina	E6861AAVIAL	-20	1 x 0.48 ml	10 µM
NEBNext Index 30 Primer for Illumina	E7716AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 37 Primer for Illumina	E7731AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 38 Primer for Illumina	E7732AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 39 Primer for Illumina	E7733AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext® Index 40 Primer for Illumina®	E7744AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 41 Primer for Illumina	E7735AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 43 Primer for Illumina	E7737AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 44 Primer for Illumina	E7738AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 45 Primer for Illumina	E7739AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 46 Primer for Illumina	E7740AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 47 Primer for Illumina	E7741AAVIAL	-20	1 x 0.04 ml	10 µM
NEBNext Index 48 Primer for Illumina	E7742AAVIAL	-20	1 x 0.04 ml	10 µM

Properties & Usage

Materials Required but not Supplied

For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.

Companion Products

Usage Guidelines

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

E7730S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.