Product Class:

NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4)
Item# Description List Price Web Price Qty
E7730L NEBNext Multiplex Oligos for Illumina (Index Primers Set 4) - 96 rxns $558.00
$502.20
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E7730S NEBNext Multiplex Oligos for Illumina (Index Primers Set 4) - 24 rxns $152.00
$136.80
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
E6040L NEBNext DNA Library Prep Master Mix Set for Illumina - 60 rxns $2,069.00
$1,862.10
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E6040S NEBNext DNA Library Prep Master Mix Set for Illumina - 12 rxns $517.00
$465.30
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E6056L NEBNext Quick Ligation Module - 100 rxns $1,773.00
$1,595.70
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E6056S NEBNext Quick Ligation Module - 20 rxns $444.00
$399.60
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E6177L NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads - 96 rxns $3,803.00
$3,422.70
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E6177S NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads - 24 rxns $1,007.00
$906.30
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E6240L NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina - 60 rxns $1,685.00
$1,516.50
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E6240S NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina - 12 rxns $422.00
$379.80
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E7103L NEBNext Ultra II DNA Library Prep with Sample Purification Beads - 96 rxns $3,570.00
$3,213.00
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E7103S NEBNext Ultra II DNA Library Prep with Sample Purification Beads - 24 rxns $934.00
$840.60
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E7370L NEBNext Ultra DNA Library Prep Kit for Illumina - 96 rxns $3,007.00
$2,706.30
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E7370S NEBNext Ultra DNA Library Prep Kit for Illumina - 24 rxns $786.00
$707.40
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E7420L NEBNext Ultra Directional RNA Library Prep Kit for Illumina - 96 rxns $5,506.00
$4,955.40
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E7420S NEBNext Ultra Directional RNA Library Prep Kit for Illumina - 24 rxns $1,697.00
$1,527.30
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E7445L NEBNext Ultra Ligation Module - 96 rxns $1,697.00
$1,527.30
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E7445S NEBNext Ultra Ligation Module - 24 rxns $528.00
$475.20
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E7530L NEBNext Ultra RNA Library Prep Kit for Illumina - 96 rxns $4,961.00
$4,464.90
ADD TO CART
E7530S NEBNext Ultra RNA Library Prep Kit for Illumina - 24 rxns $1,551.00
$1,395.90
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E7595L NEBNext Ultra II Ligation Module - 96 rxns $1,839.00
$1,655.10
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E7595S NEBNext Ultra II Ligation Module - 24 rxns $572.00
$514.80
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E7645L NEBNext Ultra II DNA Library Prep Kit for Illumina - 96 rxns $3,252.00
$2,926.80
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E7645S NEBNext Ultra II DNA Library Prep Kit for Illumina - 24 rxns $855.00
$769.50
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E7760L NEBNext Ultra II Directional RNA Library Prep Kit for Illumina - 96 rxns $4,696.00
$4,226.40
ADD TO CART
E7760S NEBNext Ultra II Directional RNA Library Prep Kit for Illumina - 24 rxns $1,383.00
$1,244.70
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E7765L NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads - 96 rxns $5,213.00
$4,691.70
ADD TO CART
E7765S NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads - 24 rxns $1,531.00
$1,377.90
ADD TO CART
E7770L NEBNext Ultra II RNA Library Prep Kit for Illumina - 96 rxns $4,474.00
$4,026.60
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E7770S NEBNext Ultra II RNA Library Prep Kit for Illumina - 24 rxns $1,317.00
$1,185.30
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E7775L NEBNext Ultra II RNA Library Prep with Sample Purification Beads - 96 rxns $4,955.00
$4,459.50
ADD TO CART
E7775S NEBNext Ultra II RNA Library Prep with Sample Purification Beads - 24 rxns $1,457.00
$1,311.30
ADD TO CART
E7805L NEBNext Ultra II FS DNA Library Prep Kit for Illumina - 96 rxns $3,476.00
$3,128.40
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E7805S NEBNext Ultra II FS DNA Library Prep Kit for Illumina - 24 rxns $920.00
$828.00
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Note: The sequence of NEBNext Index Primer 40 for Illumina has changed. Please refer to the manual titled manualE7730_0011604 if you are using Lot 0011604 for the original NEBNext Index Primer 40 for Illumina sequence (NEB #E7730)

Product Introduction

NEBNext® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. 

Additional NEBNext Multiplex Oligos products are available with sets of 12 and 96 single index primers as well as dual index primers.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing

Product Information

Description

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

For larger volume requirements, customized and bulk packaging is available by purchasing through the Custom Solutions department at NEB. Please contact [email protected] for further information.


Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (Index Primers Set 4)

E7730 workflow

    Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBNext Adaptor for Illumina -20
    USER® Enzyme -20
    NEBNext Index 30 Primer for Illumina -20
    NEBNext Index 37 Primer for Illumina -20
    NEBNext Index 38 Primer for Illumina -20
    NEBNext Index 39 Primer for Illumina -20
    NEBNext Index 40 Primer for Illumina -20
    NEBNext Index 41 Primer for Illumina -20
    NEBNext Index 43 Primer for Illumina -20
    NEBNext Index 44 Primer for Illumina -20
    NEBNext Index 45 Primer for Illumina -20
    NEBNext Index 46 Primer for Illumina -20
    NEBNext Index 47 Primer for Illumina -20
    NEBNext Index 48 Primer for Illumina -20
    Product Categories:
    Adaptors and Primers for Library Prep Products,
    ChIP-Seq Library Prep Products,
    FFPE DNA Products
    ,
    Microbiome DNA Enrichment Products
    ,
    DNA Library Prep Products
    ,
    RNA Library Prep Products
    Applications:
    DNA Library Preparation,
    Sample Prep & Target Enrichment for NGS,
    Illumina Library Preparation
    ,
    Applications of USER® and Thermolabile USER II Enzymes

    Properties & Usage

    Materials Required but not Supplied

    For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.

    Storage Temperature

    -20°C

    Protocols, Manuals & Usage

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

    Usage & Guidelines

    • Sample Sheet E7730 Kit Lot 0011604
    • Sample Sheet E7730 Kit Lot 0031704

    Faqs & Troubleshooting

    FAQs

    1. We recently made a change to the sequence of NEBNext Index Primer 40 for Illumina. Do you have an example sample sheet for the Illumina sequencer with the index names and sequences for (NEB #E7730) kit lot 0011604?
    2. We recently made a change to the sequence of NEBNext Index Primer 40 for Illumina. Do you have an example sample sheet for the Illumina sequencer with the index names and sequences for kit lot 0031704?
    3. Are NEBNext adaptor and primers for Illumina validated in Next Generation sequencing workflows?
    4. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
    5. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
    6. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
    7. Can USER Enzyme be purchased separately?
    8. Are these reagents available in a customized format? Larger format or 96 well format?
    9. Are the NEBNext Oligos for Illumina compatible with single end and paired end sequencing?
    10. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
    11. What should I do if the sample sheet from the NEB.com website is not compatible with the software version I am using?

    Troubleshooting

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.