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Ph.D.™-12 Phage Display Peptide Library Kit

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E8110S 10 png expmts
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Catalog # Size List Price Your Price
E8110S 10 png expmts
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The Ph.D.-12™ Phage Display Peptide Library Kit contains a tube of the Ph.D.-12 Phage Library, protein reagents for a control reaction and sequencing primer stocks. The Ph.D.-12 library is a combinatorial library of random 12-mer peptides fused to a minor coat protein (pIII) of M13 phage. The displayed peptide is expressed at the N-terminus of pIII. The library consists of approximately 109 electroporated (i.e., unique) sequences.

  • Ready to use complex phage library
  • Applications and methodologies are numerous and supported by over 20 years of literature citations
  • Does not require helper phage for amplification
  • Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube

The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

Citations for Phage Display



Panning with a pentavalent peptide library displayed on pIII.

Properties & Usage



Companion Products
Materials Sold Separately
References
  • Sidhu, S.S. et al. (2003). Chembiochem. 4, 14-25.
  • Ferrer, M. et al. (1999). J. Pept. Res.. 54, 32-42.
  • BouHamdan, M. et al. (1998). J. Biol. Chem.. 273, 8009-8016.
  • Whaley, S.R. et al. (2000). Nature. 405, 665-668.
  • Rozinov, M.N. and Nolan, G.P. (1998). Chem. Biol.. 5, 713-728.
  • Rodi, D.J. et al. (1999). J. Mol. Biol.. 285, 197-203.
  • Kraft, S. et al. (1999). J. Biol. Chem.. 274, 1979-1985.
  • Koolpe, M. et al. (2002). J. Biol. Chem.. 277, 46974-46979.
  • Mummert, M.E. et al. (2000). J. Exp. Med.. 192, 769-779.
  • Hetian, L. et al. (2002). J. Biol. Chem.. 277, 43137-43142.
  • White, S.J. et al. (2001). Hypertension. 37, 449-455.
  • Azzazy, H.M. and Highsmith, W.E. (2002). Clin. Biochem.. 35, 425-445.
  • Binetruy-Tournaire, R. et al. (2000). EMBO J.. 19, 1525-1533.
  • Kragler, F. et al. (2000). EMBO J.. 19, 2856-2868.
  • Gazouli, M. et al. (2002). J. Pharmacol. Exp. Ther.. 303, 627-632.
  • Romanczuk, H. et al. (1999). Hum. Gene Ther.. 10, 2615-2626.
  • Nicklin, S.A. et al. (2000). Circulation. 102, 231-237.
  • Jost, P.J. et al. (2001). FEBS Lett.. 489, 263-269.
  • Rasmussen, U.B. et al. (2002). Cancer Gene Ther.. 9, 606-612.
  • Tinoco, L.W. et al. (2002). J. Biol. Chem.. 277, 36351-36356.
  • Stratmann, J. et al. (2002). J. Clin. Microbiol.. 40, 4244-4250.
  • Mourez, M. et al. (2001). Nat. Biotechnol.. 19, 958-961.
  • Rodi, D.J. et al. (2002). Curr. Opin. Chem. Biol.. 6, 92-96.
  • Lee, L. et al. (2002). Arthritis Rheum.. 46, 2109-2120.
  • Duerr, D.M. et al. (2004). J. Virol. Methods. 116, 177-180.
  • Parmley, S.F. and Smith, G.P. (1988). Gene. 73, 305-318.
  • Berggard, T. et al. (2002). J. Biol. Chem.. 277, 41954-41959.
  • Chaudhary, J. et al. (2001). Am. J. Physiol. Cell Physiol.. 280,
  • Chen, L. and Sigler, P.B. (1999). Cell. 99, 757-769.
  • Ferrer, M. and Harrison, S.C. (1999). J. Virol.. 73, 5795-5802.
  • Biorn, A.C. et al. (2004). Biochemistry. 43, 1928-1938.
Citations
Additional Citations
  • Ohnishi T., Yanazawa M., Sasahara T., Kitamura Y., Hiroaki H., Fukazawa Y., Kii I., Nishiyama T., Kakita A., Takeda H., Takeuchi A., Arai Y., Ito A., Komura H., Hirao H., Satomura K., Inoue M., Muramatsu S., Matsui K., Tada M., Sato M., Saijo E., Shigemitsu Y., Sakai S., Umetsu Y., Goda N., Takino N., Takahashi H., Hagiwara M., Sawasaki T., Iwasaki G., Nakamura Y., Nabeshima Y., Teplow D.B., Hoshi M. (2015) Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A 112, E4465-74.PubMedID: 26224839, DOI: 10.1073/pnas.1421182112
  • Shires K, Shankland I, Mowla S, Njikan S, Jaymacker J, Novitzky N (2014) Serine and proline-rich ligands enriched via phage-display technology show preferential binding to BCR/ABL expressing cells Hematol Oncol Stem Cell Ther 7(1), 32-40.PubMedID: 24480037, DOI: 10.1016/j.hemonc.2014.01.001
  • Baumgartner J, Carillo MA, Eckes KM, Werner P, Faivre D. (2014) Biomimetic Magnetite Formation From Biocombinatorial Approaches to Mineralization Effects Langmuir 30(8), 2129-36.PubMedID: 24499323, DOI: 10.1021/la404290c
  • McGuire MJ, Gray BP, Li S, Cupka D, Byers LA, Wu L, Rezaie S, Liu YH, Pattisapu N, Issac J, Oyama T, Diao L, Heymach JV, Xie XJ, Minna JD, Brown KC (2014) Identification and Characterization of a Suite of Tumor Targeting Peptides for Non-Small Cell Lung Cancer Sci Rep 4, 4480.PubMedID: 24670678, DOI: 10.1038/srep04480
  • Kaur K, Taneja NK, Dhingra S, Tyagi JS (2014) DevR (DosR) mimetic peptides impair transcriptional regulation and survival of Mycobacterium tuberculosis under hypoxia by inhibiting the autokinase activity of DevS sensor kinase BMC Microbiol 14, 195.PubMedID: 25048654, DOI: 10.1186/1471-2180-14-195
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