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BamHI Methyltransferase

Catalog # Concentration Size List Price Quantity Your Price
M0223S 4000 units/ml 100 units $123.00
$110.70
Catalog # Size List Price Your Price
M0223S 100 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

BamHI Methyltransferase modifies the internal cytosine residue (N4) of the sequence GGATCC.
This enzyme comes from an E. coli strain that carries the cloned BamHI modification gene from Bacillus amyloliquefaciens H (ATCC 49763).

 

 
Product Source
An E. coli strain that carries the cloned BamHI modification gene from Bacillus amyloliquefaciens H (ATCC 49763).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  BamHI Methyltransferase M0223SVIAL -20 1 x 0.025 ml 4,000 units/ml
  BamHI Methyltransferase Reaction Buffer B0223SVIAL -20 1 x 1 ml 10 X
  S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by BamHI restriction endonuclease.

Reaction Conditions

1X BamHI Methyltransferase Reaction Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 37°C

Control Protection Assay

BamHI Methyltransferase is incubated with 1 µg λ DNA in 10 µl 1X BamHI Methyltransferase Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by BamHI Methyltransferase is determined by the addition of 40 µl NEBuffer 1 supplemented with 10 mM MgCl2 and 10 units of BamHI restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on agarose gels.

Storage Buffer

50 mM Tris-HCl
1 mM DTT
10 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

No

Materials Sold Separately
Notes
  • Storage of SAM: S-adenosylmethionine is stored at –20°C as 32 mM solutiondissolved in sulfuric acid (0.005 M) and 10% ethanol. SAM in this solutionstored under ideal conditions remains active for up to 6 months. SAM is unstableat (pH 7.5), 37°C, and should be replenished for reactions incubated longer than4 hours. Many problems in achieving complete digestion can be alleviated byusing fresh SAM.
References
  • Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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