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Mung Bean Nuclease

Catalog # Concentration Size List Price Quantity Your Price
M0250L 10000 units/ml 7500 units $438.00
$394.20
M0250S 10000 units/ml 1500 units $109.00
$98.10
Catalog # Size List Price Your Price
M0250L 7500 units $438.00
$394.20
M0250S 1500 units $109.00
$98.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Single-stranded (ssDNA or RNA) specific endonuclease
  • Catalyzes the removal of single-stranded extension in double-stranded DNA
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Mung Bean can be used for:

  • Removal of both 3' and 5' single-stranded overhangs from double-stranded DNA to create blunt ends
  • Cleavage of single-stranded DNA and RNA
  • Cleavage of the single-stranded region in a DNA hairpin
  • Mapping of RNA transcripts
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    DNA Blunting Tutorial
  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?

 

Product Source
Mung bean sprouts.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Mung Bean Nuclease M0250SVIAL -20 1 x 0.15 ml 10,000 units/ml
  Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X
  Mung Bean Nuclease M0250LVIAL -20 1 x 0.75 ml 10,000 units/ml
  Mung Bean Nuclease Reaction Buffer B0250SVIAL -20 1 x 1 ml 10 X
Application Features
  • Removal of 3´ and 5´ extensions or single-stranded regions from DNA or RNA termini
  • Transcriptional mapping
  • Cleavage of hairpin loops
  • Excision of gene coding sequences from genomic DNA
  • Generation of new restriction sites

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of M13mp18 single-stranded DNA to fragments less than 1 kb in length in a total reaction volume of 80 µl in 1X Mung Bean Nuclease Reaction Buffer when incubated for 15 minutes at 37°C.

Reaction Conditions

1X Mung Bean Nuclease Reaction Buffer
Incubate at 30°C

1X Mung Bean Nuclease Reaction Buffer
30 mM NaCl
50 mM sodium acetate
1 mM ZnSO4
(pH 5 @ 25°C)

Storage Buffer

10 mM sodium acetate
0.1 mM Zinc Acetate
1 mM Cysteine
50% Glycerol
0.001% Triton® X-100
pH 5 @ 25°C

Molecular Weight

Theoretical: 39 kDa


Companion Products
Materials Sold Separately
Notes
  • It is no longer necessary to supplement Mung Bean Nuclease reactions with Zn2+. The zinc acetate in the storage buffer fulfills the Zn2+ requirement of the enzyme even after dilution in a reaction.
  • Do not attempt to heat inactivate, DNA will “breathe” before enzyme inactivates, causing undesirable degradation. 
References
  • Kowalski, D. et al. (1976). Biochemistry. 15, 4457-4463.
  • McCutchan, T.F. et al. (1984). Science. 225, 626-628.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
Effective May 2, 2022, updated unit definition.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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