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Therminator™ DNA Polymerase

Catalog # Concentration Size List Price Quantity Your Price
M0261L 2000 units/ml 1000 units $695.00
$625.50
M0261S 2000 units/ml 200 units $174.00
$156.60
Catalog # Size List Price Your Price
M0261L 1000 units $695.00
$625.50
M0261S 200 units $174.00
$156.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Therminator DNA Polymerase is a 9°N™ DNA Polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides (1,2).

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Enhanced incorporation of modified nucleotides
  • Single base incorporation of dideoxy and acyclonucleotides

Highlights

 

Product Source
An E. coli strain that carries the 9°N (D141A / E143A / A485L) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from Thermococcus species 9°N-7.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Therminator™ DNA Polymerase M0261SVIAL -20 1 x 0.1 ml 2,000 units/ml
  ThermoPol® Reaction Buffer B9004SVIAL -20 1 x 1.5 ml 10 X
  Therminator™ DNA Polymerase M0261LVIAL -20 1 x 0.5 ml 2,000 units/ml
  ThermoPol® Reaction Buffer B9004SVIAL -20 3 x 1.5 ml 10 X
Application Features
  • DNA sequencing by partial ribosubstitution (3)
  • DNA sequencing using dideoxy (4) or acyclo (5) chain terminators
  • SNP analysis with dideoxy or acyclo chain terminators (6)

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X ThermoPol® Reaction Buffer

1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 90000 daltons

5' - 3' Exonuclease

No

3' - 5' Exonuclease

No

Strand Displacement

+

Unit Assay Conditions

1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed single-stranded M13mp18.

Companion Products
Materials Sold Separately
Notes
  • Amplification of extended regions may require optimization of reaction conditions.
References
  • Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2555.
  • Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res. 30, 605-613.
  • Barnes, W.F. (1978). J. Mol. Biol.. 119, 83-99.
  • Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
  • Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
  • Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
Citations
Publications
  • Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019). Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci. 6, 28.PubMedID: 31069234​, DOI: 10.3389/fmolb.2019.00028
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
9°N™ and THERMINATOR™ are trademarks of New England Biolabs, Inc.

SEQUENASE™ is a trademark of GE Healthcare.

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