Product Class: Other

RNase H
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Item# Description List Price Web Price Qty
M0297L RNase H - 1,250 units $401.00
$360.90
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M0297S RNase H - 250 units $100.00
$90.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Product Introduction

RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA.

  • Recombinant enzyme supplied with Reaction Buffer
  • Tested for the absence of endonucleases, ss-DNase and other RNases
  • This enzyme does not digest single or double-stranded DNA

Product Information

Description

RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.

Product Source

An E. coli strain that carries the cloned RNase H gene (rnh) from Escherichia coli.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
RNase H Reaction Buffer -20 10 X
Product Categories:
RNases Products,
cDNA Synthesis & Reverse Transcriptases Products
Applications:
RT-PCR & cDNA Synthesis,
RT-qPCR, RT-PCR and cDNA Synthesis,
PCR

Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 37°C.

    Reaction Conditions

    1X RNase H Reaction Buffer
    Incubate at 37°C

    1X RNase H Reaction Buffer
    50 mM Tris-HCl
    3 mM MgCl2
    10 mM DTT
    (pH 8.3 @ 25°C)

    Storage Temperature

    -20°C

    Storage Conditions

    50 mM KCl
    10 mM Tris-HCl
    0.1 mM EDTA
    1 mM DTT
    200 µg/ml BSA
    50% Glycerol
    pH 7.4 @ 25°C

    Heat Inactivation

    65°C for 20 min

    References

    1. Davis, R. et al. (1988). Cell Biol. 8, 4745-4755.
    2. Gubbler, U. and Hoffman, B.J. (1983). Gene. 25, 263-269.
    3. Donnis-Keller, H. (1979). Nucl. Acids Res. 7, 179.
    4. Goodwin, E.C. and Rottman, F.M. (1992). Nucl. Acids Res. 20, 916.

    Protocols, Manuals & Usage

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

    Faqs & Troubleshooting

    FAQs

    1. When I thaw the 10X RNase H Reaction Buffer, I see a white precipitate.  What is it and what should I do about it?
    2. Is DNA Polymerase I active in RNase H buffer?
    3. Which side of the RNA base does RNase H cut?
    4. What is the activity of RNase H at 30°C?

    Troubleshooting

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.