hSMUG1

Catalog # Concentration Size List Price Quantity Your Price
M0336S 5000 units/ml 500 units $123.00
$110.70
Catalog # Size List Price Your Price
M0336S 500 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Human single-stranded selective monofunctional uracil DNA glycosylase
  • Monofunctional DNA glycosylase that catalyzes the hydrolysis of the N-glycosidic bond to release uracil and uracil-derivatives from DNA
  • Major substrates include uracil, 5-hydroxyuracil, 5-hydroxymethyluracil, and 5-formyluracil
  • Active on ss and dsDNA
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Highlights
 
Product Source
An E. coli strain which carries the cloned human SMUG1 gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  hSMUG1 M0336SVIAL -20 1 x 0.1 ml 5,000 units/ml
  NEBuffer™ 1 B7001SVIAL -20 1 x 1.25 ml 10 X
  Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to excizse 1 pmol of deoxyuracil from a 34 mer oligonucleotide duplex containing a single dU site in a total reaction volume of 10 µl in 1 hour at 37°C.

Reaction Conditions

1X NEBuffer™ 1
Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C

1X NEBuffer™ 1
10 mM Bis-Tris-Propane-HCl
10 mM MgCl2
1 mM DTT
(pH 7 @ 25°C)

Storage Buffer

10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 minutes

Molecular Weight

Theoretical: 29861 daltons

Unit Assay Conditions

1X NEBuffer 1 supplemented with 100 µg/ml Recombinant Albumin containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 µl. After release of dU, the oligonucleotide is cleaved by treating the resulting AP site with 100 mM NaOH for 10 minutes at 80°C.

Notes
  • hSMUG1 has 50% activity on 5-hydroxymethyluracil when compared to uracil. hSMUG1 has 50% activity on ssDNA compared to ds DNA.
References
  • Cannon-Crlson, S.V. et al. (1989). J. Biol. Chem. 264, 13306-13312.
  • Masaoka, A. et al. (2003). Biochemistry. 42, 5003-5012.
  • Wibley, J.E.A. et al. (2003). Cell. 11, 1647-1659.
  • Singh, N. et al. (1998). Experimental Cell  Research. 175, 184-191.
  • Collins, A. et al. (1993). Carcinogenesis . 14, 1733-1735..
  • Collins, A. et al. (1996). Environmental Health Perspectives. 104, 465-469.
Usage Guidelines
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications
Recombinant Albumin (rAlbumin) replaces BSA beginning with Lot #10112346.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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