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Bst 2.0 DNA Polymerase

Catalog # Concentration Size List Price Quantity Your Price
M0537L 8000 units/ml 8000 units $468.00
$421.20
M0537M 120000 units/ml 8000 units $468.00
$421.20
M0537S 8000 units/ml 1600 units $117.00
$105.30
Catalog # Size List Price Your Price
M0537L 8000 units $468.00
$421.20
M0537M 8000 units $468.00
$421.20
M0537S 1600 units $117.00
$105.30
Catalog #
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Bst 2.0 DNA Polymerase displays improved amplification speed, yield and salt tolerance

  • Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP)
  • Improved amplification reaction properties compared to Bst DNA Polymerase
  • Fast polymerization
  • Flexible reaction conditions including a higher salt tolerance and thermostability, as compared to wild-type Bst DNA Polymerase, Large Fragment (NEB #M0275)
  • WarmStart® version available for increased reaction efficiency and room temperature setup (NEB #M0538)
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Bst 2.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment). Bst 2.0 DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.


LAMP Salt Tolerance LAMP Salt Tolerance

Performing LAMP reactions with increasing amounts of salt demonstrate increased resistance to inhibitors by using Bst 2.0. Bst 2.0 maintains high levels of activity where wild-type Bst DNA polymerase, large fragment, is completely inhibited.
Rapid LAMP Detection Rapid LAMP Detection

LAMP reactions amplifying different targets demonstrate a more rapid amplification threshold time using Bst 2.0 vs. Bst, large fragment.
Product Source
Bst 2.0 DNA Polymerase is prepared from an E. coli strain that expresses the Bst 2.0 DNA Polymerase protein from an inducible promoter.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Bst 2.0 DNA Polymerase M0537SVIAL -20 1 x 0.2 ml 8,000 units/ml
  Isothermal Amplification Buffer Pack B0537SVIAL -20 1 x 1.5 ml 10 X
  Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM
  Bst 2.0 DNA Polymerase M0537LVIAL -20 1 x 1 ml 8,000 units/ml
  Isothermal Amplification Buffer Pack B0537SVIAL -20 2 x 1.5 ml 10 X
  Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM
  Bst 2.0 DNA Polymerase M0537MVIAL -20 1 x 0.067 ml 120,000 units/ml
  Isothermal Amplification Buffer Pack B0537SVIAL -20 2 x 1.5 ml 10 X
  Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM
Application Features
  • Isothermal amplification (LAMP)
  • Applications requiring strand-displacement DNA synthesis
  • DNA sequencing through high GC regions
  • Rapid sequencing from nanogram amounts of DNA template

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Reaction Conditions

1X Isothermal Amplification Buffer Pack
Incubate at 65°C

1X Isothermal Amplification Buffer Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
50 mM KCl
2 mM MgSO4
0.1% Tween® 20
(pH 8.8 @ 25°C)

Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.1 @ 25°C

Heat Inactivation

80°C for 20 minutes

Unit Assay Conditions

50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [3H]-dTTP and 100 μg/ml BSA.

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Notes
  • Bst 2.0 DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.
  • Reaction temperatures above 70°C are not recommended.
  • Bst 2.0 DNA Polymerase cannot be used for thermal cycle sequencing or PCR.
  • Specific reaction conditions will vary for different isothermal amplification applications. For best results, use 1X Isothermal Amplification Buffer.
Citations
Additional Citations
  • Poole, C.B., Ettwiller, L., Tanner, N.A., Evans, T.C., Jr., Wanji, S.,Carlow, C.K. (2015) Genome filtering for new DNA biomarkers of Loa loa infection suitable for loop-mediated isothermal amplification. PLoS One 10(9), e0139286..PubMedID: 23272258, DOI: 10.1371/journal.pntd.0001948
  • Sanchita Bhadra, Yu Sherry Jiang, Mia R Kumar, Reed F Johnson, Lisa E Hensley, Andrew D Ellington (2015) Real-Time Sequence-Validated Loop-Mediated Isothermal Amplification Assays for Detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV). PLoS One 10, e0123126.PubMedID: 25856093, DOI: 10.1371/journal.pone.0123126
Publications
  • Tanner NA, Zhang Y, Evans TC Jr (2015). Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes. Biotechniques. Feb, 59-68. DOI: 10.2144/000114253
  • Poole CB, Zhang Y, Evans TC Jr., Carlow CK (2012). Diagnosis of brugian filariasis by loop-mediated isothermal amplification. PLoS Negl Trop Dis. 6(12), e1948..PubMedID: 2327225, DOI: 10.1371/journal.pntd.0001948
  • Tanner NA, Zhang Y, Evans TC Jr. (2012). Simultaneous multiple target detection in real-time loop-mediated isothermal amplification Biotechniques. Aug, 81-9.PubMedID: 23030060, DOI: 10.2144/0000113902
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.The purchase of NEB Bst products conveys to the purchaser the limited, nontransferable right to use the purchased products to perform loop-mediated isothermal amplification ("LAMP") for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd. and any use other than research may require a license from Eiken Chemical Co., Ltd. A patent is pending for NEB's Bst DNA Polymerase.NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs Inc.
WARMSTART™ is a trademark of New England Biolabs, Inc.

TRITON® is a registered trademark of Union Carbide Corporation.
TWEEN® is a registered trademark of Uniqema Americas LLC.

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