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Exonuclease VIII, truncated

Catalog # Concentration Size List Price Quantity Your Price
M0545S 10000 units/ml 1000 units $119.00
$107.10
Catalog # Size List Price Your Price
M0545S 1000 units $119.00
$107.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Double-stranded DNA specific exonuclease
  • Initiates at the 5' termini of linear double-stranded DNA
  • Catalyzes the removal of nucleotides from linear double-stranded DNA in the 5' to 3' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease VIII, truncated can be used for:

  • Removal of linear double-stranded DNA, leaving behind circular DNA in the sample
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?
Product Source
An E. coli strain that carries a plasmid with genetic engineering active domain of Exonuclease VIII.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Exonuclease VIII, truncated M0545SVIAL -20 1 x 0.1 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
Application Features
•    Degradation of linear dsDNA while maintaining double stranded circular DNA.

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H] DNA.

Reaction Conditions

1X NEBuffer™ 4
Incubate at 37°C

1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

50 mM Tris-HCl
100 mM NaCl
0.1 mM EDTA
1 mM DTT
0.1% Triton® X-100
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

70°C for 15 minutes

Unit Assay Conditions

1X NEBuffer 4 with 0.15 mM with sonicated duplex 3H- DNA.

References
  • Chang, et al. (2001). J. Biol. Chem.. 46004–46010.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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