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Hi-T7® RNA Polymerase

Catalog # Concentration Size List Price Quantity Your Price
M0658S 50000 units/ml 5000 units $129.00
$116.10
Catalog # Size List Price Your Price
M0658S 5000 units $129.00
$116.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Hi-T7 RNA Polymerase is designed for in vitro transcription of RNA at higher temperatures.

  • Supplied with 10X Hi-T7 RNA Polymerase Reaction Buffer formulated for optimal performance
  • Active from 37-56°C
  • For standard in vitro transcription at 37°C use T7 RNA Polymerase (NEB# M0251)
  • Increased co-transcriptional capping efficiency with cap analogs
  • Decreased immunogenicity from RNA synthesized at higher temperature due to reduced dsRNA by-product formation
  • High concentration format also available (NEB #M0470)
  • Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.

Hi-T7 RNA Polymerase is an engineered DNA-dependent RNA polymerase that is highly specific for T7 phage promoters and is designed to function at higher temperatures than the wild-type bacteriophage T7 RNA Polymerase. Hi-T7 RNA Polymerase functions at an optimal temperature of 50-52°C.

Product Source
An E. coli strain that carries an engineered His-tag T7 RNA Polyermase gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Hi-T7® RNA Polymerase M0658SVIAL -20 1 x 0.1 ml 50,000 units/ml
  Hi-T7® RNA Polymerase Reaction Buffer B0658AVIAL -20 1 x 1 ml
Application Features
  • Radiolabeled RNA probes
  • Non-isotopic RNA labeling
  • Guide RNA for gene targeting
  • mRNA for in vitro translation, microinjection and transfection 
  • RNA structure, processing and catalysis studies
  • RNA amplification
  • Isothermal amplification
  • Anti-sense RNA for gene expression
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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