Product Class: Other

Nuclease P1
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Item# Description List Price Web Price Qty
M0660S Nuclease P1 - 10,000 units $71.00
$63.90
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

    Product Introduction

    • Single-stranded (ssDNA or RNA) specific endonuclease

    Nuclease P1 is ideal for:

    • Conversion of single-stranded DNA or RNA to 5'-mononucleotides
    • Analysis of the base composition of nucleic acids
    • Studies of the potential damage and modification in DNA
     

    Product Information

    Description

    Nuclease P1 (from P. citrinum) is a zinc-dependent single-strand specific nuclease which hydrolyzes 3′->5′ phosphodiester bonds in RNA and ssDNA with no base specificity. Nuclease P1 also exhibits 3′-phosphomonoesterase activity (1). Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer 1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.

    Product Source

    Isolated from Penicillium citrinum.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    Nuclease P1 Reaction Buffer -20 10X
    Product Categories:
    Nucleases Products

    Properties & Usage

      Unit Definition

      One unit is defined as the amount of enzyme required to liberate 1.0 µg of acid soluble nucleotides from Torula Yeast total RNA per min at 37°C in 1X Nuclease P1 Reaction Buffer.

      Reaction Conditions

      1X Nuclease P1 Reaction Buffer
      Incubate at 37°C

      1X Nuclease P1 Reaction Buffer
      50 mM sodium acetate
      (pH 5.5 @ 25°C)

      Storage Temperature

      -20°C

      Storage Conditions

      25 mM Tris-HCl
      50 mM NaCl
      1 mM ZnCl2
      50% Glycerol
      pH 7.2 @ 25°C

      Heat Inactivation

      75°C for 10 min

      Product Notes

      1. Substrate specificity for Nuclease P1 is as follows: 3´ AMP > RNA > ssDNA >> dsDNA (1).
      2. The rate of hydrolysis of 2´-AMP is 3,000-fold less than that of 3´-AMP (1).
      3. Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer 1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.

      References

      1. Fujimoto, M., Kuninaka, A. and Yoshino, H. (1974). Agric. Biol. Chem. 38, 777-783.

      Protocols, Manuals & Usage

      Protocols

      1. Protocol for #M0660

      Faqs & Troubleshooting

      FAQs

      1. Is Nuclease P1 sensitive to salt?
      2. Can Nuclease P1 be inactivated?
      3. Does Nuclease P1 require ZnCl2?
      4. What applications is Nuclease P1 best suited for?
      5. What substrates does Nuclease P1 digest?
      6. How much ssDNA or RNA can Nuclease P1 digest?
      7. What is the activity of Nuclease P1 in other buffers?
      8. How does the new unit definition relate to the historical unit definition for Nuclease P1?
      9. Will Nuclease P1 degrade double stranded DNA (dsDNA)?

      Troubleshooting

      Quality, Safety & Legal

      Quality Assurance Statement

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

      Specifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

      Certificate Of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

      Safety DataSheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

      Legal and Disclaimers

      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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