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ET SSB

Catalog # Concentration Size List Price Quantity Your Price
M2401S 500 µg/ml 50 µg $265.00
$238.50
Catalog # Size List Price Your Price
M2401S 50 µg $265.00
$238.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

  • Improve the processivity of DNA polymerase
  • Stabilization and marking of ssDNA structure
  • Increase the yield and specificitiy of PCR
  • Increase the yield and processivity of RT during RT-PCR
  • Improve DNA sequencing through regions with strong secondary structure
ET SSB (Extreme Thermostable Single-Stranded DNA Binding Protein) is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism, which remains fully active after incubation at 95°C for 60 minutes. Due to the extreme thermostability, ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

Figure 1: Improved multiplex PCR by ET SSB. Figure 1: improved multiplex PCR by ET SSB

By adding ET SSB, specific amplification was accomplished in PCR using one to five primer pairs in incurring order (lanes 1-5). Lane 5C shows a PCR reaction using 5 primer pairs, with no ET SSB included (control reaction). Lane M: 100 bp DNA Ladder.
Figure 2: Multiplex HDA was improved by ET SSB. Figure 2: Multiplex HDA was improved by ET SSB

The HDA reactions were prepared with either single or two sets of primers to amplify the invE (A) and/or invA (B) from Salmonella typhimurium genomic DNA. Lane M: Low Molecular DNA Ladder; Lane A: invE gene; Lane B: invA gene; Lane AB: invE and invA genes with no ET SSB present; Lane AB**: invE and invA genes with ET SSB in the HDA reaction. ** the reaction contained ET SSB.
Product Source
An E. coli strain that carries the cloned ssb gene from a hyperthermophilic organism
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  ET SSB M2401SVIAL -20 1 x 0.1 ml 500 µg/ml

Properties & Usage

Unit Definition

Sold by mass of pure protein as determined by OD280.

Storage Buffer

10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Apparent: 16 kDa


Notes
  • ET SSB is active in any polymerase buffer. Add 200 ng of ET SSB per 50 µl reaction.
References
  • Myers, T.W. and Romano, L.J. (1988). J. Biol. Chem. 263, 17006-17015.
  • Reddy, M.S. (2000). Biochemistry. 39, 14250-14262.
  • West, S.C., Cassuto, E. and Howard-Flanders, P. (1982). Mol. Gen. Genet. 186, 333-338.
  • Goldmeyer, J., Kong, H., and Tang, W. (2007). J. Mol. Diagnostics. 9, 639-644.
  • Delius, H., Mantell, N.J. and Alberts, B. (1972). J. Mol. Biol. 67, 341-350.
  • Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1990). Nucl. Acids Res. 18, 1079.
  • Chou, Q. (1992). Nucl. Acids Res. 20, 4371.
  • Oshima, R.G. (1992). Biotechniques. 13, 188.
  • Rapley, R. (1994). Mol. Biotechnol. 2, 295-298.
  • Olszewski, M. et al. (2005). Mol. Cell Probes. 19, 203-205.
  • Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
  • Villalva, C. et al. (2001). Biotechniques. 31, 81-83, 86.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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