Product Class: Other

TriDye™ 100 bp DNA Ladder
Item# Description List Price Web Price Qty
N3271S TriDye 100 bp DNA Ladder - 125 gel lanes $126.00
$113.40
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
N0467L Quick-Load 100 bp DNA Ladder - 375 gel lanes $290.00
$261.00
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N0467S Quick-Load 100 bp DNA Ladder - 125 gel lanes $117.00
$105.30
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N0468L Quick-Load 1 kb DNA Ladder - 375 gel lanes $210.00
$189.00
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N0468S Quick-Load 1 kb DNA Ladder - 125 gel lanes $88.00
$79.20
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N3231L 100 bp DNA Ladder - 500 gel lanes $318.00
$286.20
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N3231S 100 bp DNA Ladder - 100 gel lanes $80.00
$72.00
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N3232L 1 kb DNA Ladder - 1,000 gel lanes $298.00
$268.20
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N3232S 1 kb DNA Ladder - 200 gel lanes $76.00
$68.40
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N3234L PCR Marker - 500 gel lanes $370.00
$333.00
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N3234S PCR Marker - 100 gel lanes $92.00
$82.80
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N3236L 50 bp DNA Ladder - 500-1000 gel lanes $387.00
$348.30
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N3236S 50 bp DNA Ladder - 100-200 gel lanes $96.00
$86.40
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N3270S TriDye 1 kb Plus DNA Ladder - 250 gel lanes $112.00
$100.80
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N3272S TriDye 1 kb DNA Ladder - 125 gel lanes $92.00
$82.80
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T1010L Monarch Plasmid Miniprep Kit - 250 preps $407.00
$366.30
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T1010S Monarch Plasmid Miniprep Kit - 50 preps $98.00
$88.20
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T1020L Monarch DNA Gel Extraction Kit - 250 preps $570.00
$513.00
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T1020S Monarch DNA Gel Extraction Kit - 50 preps $124.00
$111.60
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T1030L Monarch PCR & DNA Cleanup Kit - 250 preps $570.00
$513.00
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T1030S Monarch PCR & DNA Cleanup Kit - 50 preps $124.00
$111.60
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    Product Introduction

    • Size range: 100 bp to 1,517 bp
    • Convenient, ready-to-load format
    • Formulated with 3 different tracking dyes
    • Easy to identify reference bands
    • Suitable for 125 gel lanes

     

    Product Information

    Description

    TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

    The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points.

    • Recommended gel percentage range: 1.2-3%
    • Optimum separation on 2%
    TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.

    N3271fig2_thumb
    100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel. Mass values are for 0.5 µg/lane
    Product Categories:
    DNA Markers & Ladders Products
    Applications:
    DNA Analysis

    Properties & Usage

      Bases

      Fragment Mass bp
      1 45 1,517
      2 35 1,200
      3 95 1,000
      4 27 900
      5 24 800
      6 21 700
      7 18 600
      8 97 500/517
      9 38 400
      10 29 300
      11 25 200
      12 48 100

      Effective Size Range

      100.0bp to 1,517.0bp

      Storage Temperature

      4°C

      Storage Conditions

      0.006% Xylene Cyanol
      10 mM Tris-HCl
      10 mM EDTA
      10% Glycerol
      0.006% bromophenol blue
      0.06% Orange G
      pH 7.9 @ 25°C

      Product Notes

      1. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
      2. We recommend loading 10 μl (0.5 μg) of TriDye DNA Ladder per gel lane.
      3. In general, it is recommended to load 1ul per mm of gel lane.
      4. TriDye DNA Ladder is stable for at least 6 months at 25°C
      5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

      References

      1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

      Protocols, Manuals & Usage

      Protocols

      1. Protocol for TriDye 100 bp DNA Ladder (NEB #N3271)

      Datacards

      The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

      Tools & Resources

      Selection Charts

      • DNA Markers & Ladders Selection Chart

      Faqs & Troubleshooting

      FAQs

      1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
      2. How can I quantify the amount of DNA in each band of a marker?
      3. Why are there extra bands visible on polyacrylamide gels?
      4. What are the differences in the four versions of the 1 kb Plus (formerly called the 2-Log DNA Ladder), 100 bp and 1 kb DNA Ladders that NEB sells?
      5. Can I use GelRed® with the DNA Ladders from NEB?
      6. Can I use Midori Green with the DNA Ladders from NEB?
      7. Can I use SYBR® with the DNA Ladders from NEB?

      Troubleshooting

      Tech Tips

      Mix well before use, especially after thawing.

      Quality, Safety & Legal

      Quality Assurance Statement

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

      Specifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

      Certificate Of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

      Safety DataSheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

      Legal and Disclaimers

      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.