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M13mp18 Single-stranded DNA

Catalog # Concentration Size List Price Quantity Your Price
N4040S 250 µg/ml 10 µg $70.00
$63.00
Catalog # Size List Price Your Price
N4040S 10 µg $70.00
$63.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • View sequence details
  • Single stranded, circular viral DNA from M13mp18
  • Useful as a template in the dideoxy-nucleotide termination sequencing method
The single-stranded viral DNA is isolated from M13mp18. M13mp18 is a M13 lac phage vector which contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the gene encoding β-Galactosidase.This DNA preparation is useful as a standard and has been tested as a template in the dideoxy-nucleotide termination method of sequencing DNA.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  M13mp18 Single-stranded DNA N4040SVIAL -20 1 x 0.04 ml 250 µg/ml

Properties & Usage

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C



Companion Products
Notes
  • M13mp18 DNA may occur in two different forms:

    1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
    2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.
    Generally single-stranded DNAs are not cut by restriction enzymes.
References
  • Messing, J. (1983). R. Wu(Ed.), Methods Enzymol.. 101, 20. San Diego: Academic Press.
FAQs
Tech Tips

  • M13mp18 DNA may occur in two different forms:

    1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
    2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.
    Generally single-stranded DNAs are not cut by restriction enzymes.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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