Product Class: Other

IdeZ Protease (IgG-specific)
icon_cloned icon_recomb icon_incTemp37 icon_HI_65
Item# Description List Price Web Price Qty
P0770S IdeZ Protease (IgG-specific) - 4,000 units $600.00
$540.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Product Introduction

IdeZ Protease (IgG-specific) is a recombinant antibody specific protease that recognizes all human, sheep, monkey, and rabbit IgG subclasses, cleaving specifically at a single recognition site below the hinge region, to yield a homogenous pool of F(ab´)2 and Fc fragments. IdeZ Protease more effectively cleaves murine IgG2a than IdeS.

  • Complete fragmentation of antibodies and immunoglobulin fusion proteins in 30 minutes under native conditions
  • Supplied in solution; optimal activity and stability for up to 24 months
  • Combine with PNGase F for complete fragmentation and deglycosylation
  • Recombinant enzyme with no detectable non-specific protease contaminating activities
  • Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
  • ≥95% purity, as determined by SDS-PAGE and intact ESI-MS

Product Information

Description


 
IdeZ Protease (IgG-specific) is a recombinant antibody specific protease cloned from Streptococcus equi subspecies zooepidemicus that recognizes all human, sheep, monkey, and rabbit IgG subclasses, specifically cleaving at a single recognition site below the hinge region, yielding a homogenous pool of F(ab´)2 and Fc fragments. IdeZ Protease more effectively cleaves murine IgG2a than IdeS.

Digestion of lgG with IdeZ Protease (lgG-specific), followed by denaturation.

Product Source

Cloned from Streptococcus equi subspecies zooepidemicus and expressed in E. coli. 

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration
GlycoBuffer 2 -20 10 X
Product Categories:
Proteases Products,
Proteome Analysis Products
Applications:
Proteomics

Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to cleave > 95% of 1 µg of human IgG, in 15 minutes at 37°C in a total reaction volume of 10 µl.

    Storage Temperature

    -20°C

    Storage Conditions

    20 mM Tris-HCl
    50 mM NaCl
    1 mM EDTA
    pH 7.5 @ 25°C

    Heat Inactivation

    65°C for 10 min

    Molecular Weight

    Theoretical: 35578 daltons

    Unit Assay Conditions

    Two fold dilutions of IdeZ Protease (IgG-specific) are incubated with 1 μg of human IgG and 1X GlycoBuffer 2 in a 10 µl reaction. The reaction mix is incubated for 15 minutes at 37°C. Separation of reaction products are visualized by SDS-PAGE.

    Storage Notes

    • Avoid repeated freeze/thaw cycles.

    Product Notes

    1. Store at -20°C for up to two years.
    2. IdeZ Protease efficiently cleaves human, humanized, chimeric, sheep,rabbit and monkey IgG as well as mouse IgG2a and IgG3. IdeZ Proteasewill also cleave Fc-fusion proteins, such as Enbrel.
    3. IdeZ Protease does not cleave mouse IgG1 or IgG2b, rat, porcine, bovineor goat IgG. It also does not cleave non-IgG isotypes including IgA,IgM, IgD and IgE.

    Protocols, Manuals & Usage

    Protocols

    1. Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)
    2. Reaction conditions for Simultaneous Digestion of IgG with IdeZ Protease (IgG-specific) and PNGase F (fragmentation and deglycosylation) (P0770)

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

    Faqs & Troubleshooting

    FAQs

    1. What is the advantage of IdeZ over IdeS?
    2. Is IdeZ Protease active in Phosphate Buffered Saline (PBS)?
    3. Can protein A magnetic beads (NEB# S1425S) be used to create Fc and Fab fragment pools after cleavage with IdeZ Protease?
    4. Can IdeZ Protease cleave IgGs other than human?
    5. Can IdeZ Protease cleave Ig isotypes other than IgG?
    6. Can PNGase F be used together with IdeZ Protease under native conditions to deglycosylate the Fc portion of an antibody?
    7. What is the cleavage site for IdeZ Protease (IgG-specific)?

    Troubleshooting

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

    While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

    For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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