Product Class: Other

SNAP-Surface® 649
Item# Description List Price Web Price Qty
S9159S SNAP-Surface 649 - 50 nmol $447.00
$402.30
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

    Product Introduction

    SNAP-Surface 649 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins on cell surfaces and in solution.

    • This is a non-cell-permeable substrate (BG-649) based on the Dyomics dye DY-649P1
    • It is suitable for excitation with a 650 nm diode laser or use with Cy5 filter sets
    • It has an excitation maximum at 655 nm and emission maximum at 676 nm

    Product Information

    Description

    This package includes 50 nmol of SNAP-Surface 649 substrate, sufficient to make 10 ml of a 5 μM SNAP-tag fusion protein labeling solution.

    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine- DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAPtag substrate is described below.

    Excitation (dotted line) and emission spectra of SNAP-Surface 649 coupled to SNAP-tag in buffer at pH 7.5Excitation (dotted line) and emission spectra of SNAP-Surface 649 coupled to SNAP-tag in buffer at pH 7.5
    9147a_thumb
    Structure of SNAP-Surface 649 (MW 1221.3 g/mol).
    Product Categories:
    SNAP-tag Substrates Products ,
    Protein Labeling Products
    Applications:
    Snap Surface Products,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling Snap Clip

    Properties & Usage

      Materials Required but not Supplied

      • Cells expressing SNAP-fusion proteins. Proteins of interest can be expressed with the SNAP-tag as either an N- or a C-terminal fusion, but note that the tag needs to be exposed to the extracellular surface of the plasma membrane for labeling with SNAPSurface 649.
      • Tissue culture materials and media
      • Transfection reagents
      • Fluorescence microscope with suitable filter set
      • DMSO

      Excitation

      655nm

      Emission

      676nm

      Storage Temperature

      -20°C

      Product Notes

      1. Storage: SNAP-Surface 649 should be stored at –20°C (longterm) or at 4°C in the dark (short term, less than4 weeks). Protect the substrate from light and moisture.With proper storage at –20°C the substrateshould be stable for at least three years dry or3 months dissolved in DMSO.

      Protocols, Manuals & Usage

      Protocols

      1. Cellular Labeling (S9159)
      2. Labeling of Proteins in vitro (S9159)
      3. View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)

      Datacards

      The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)

      Faqs & Troubleshooting

      FAQs

      1. Cellular Imaging and Analysis FAQs

      Troubleshooting

      Quality, Safety & Legal

      Quality Assurance Statement

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

      Specifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

      Certificate Of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

      Safety DataSheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

      Legal and Disclaimers

      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      Licenses

      Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938. For detailed information:  see www.neb.com/cia/legal.

      The products and/or their use may be covered by one or more of the following patents and patent applications:
      Methods for Using O6-Alkylguanine-DNA-Alkyltransferases: US 7,939,284; US 8,367,361; EP 1 410 023; EP 2 037 271; EP 1 696 234; EP 2 211 177; JP 4195815; JP 4226053; CN 1295510; CN 1975422; and SG 100125.

      Substrates for O6-Alkylguanine-DNA Alkyltransferase: US 7,799,524; and JP 4976651.

      Mutants of O6-Alkylguanine-DNA-Alkyltransferases: US 7,888,090. EP 1720981 (pending).

      Specific Substrates for O6-Alkylguanine-DNA-Alkyltransferases: US 8,163,479. EP 1730298 (pending).