Monarch® DNA Gel Extraction Kit

Catalog # Concentration Size List Price Quantity Your Price
T1020L 250 preps $664.00
$597.60
T1020S 50 preps $152.00
$136.80
Catalog # Size List Price Your Price
T1020L 250 preps $664.00
$597.60
T1020S 50 preps $152.00
$136.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Quickly and easily purify DNA from agarose gels with high yields.

  • Elute in as little as 6 μl
  • Prevent buffer retention and salt carry-over with optimized column design
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits 
  • Responsibly-sourced and recyclable packaging

 

Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.

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The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging


View our videos on protocols, tips, and recycling Monarch.

Monarch DNA Cleanup Column Design
Monarch DNA Cleanup Column Design
Monarch columns are designed for performance
Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.

Advantages:
  • Elute in as little as 6 μl
  • Prevent buffer retention and salt carry-over with optimized column design
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging

Specifications

DNA Sample Type: double-stranded DNA from agarose gels
Binding Capacity: up to 5 μg
DNA Size Range: ~50 bp to 25 kb
Typical Recovery: DNA (50 bp to 10 kb): 70–90%
DNA (11–23 kb): 50–70%
Elution Volume: ≥ 6 μl
Purity: A260/280 > 1.8
Protocol Time: 10 minutes of spin and incubation time
Compatible Downstream
Applications:
ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.

Monarch DNA Gel Extraction Kit Protocol 
Monarch DNA Gel Extraction Kit Protocol
DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications
 DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications. One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume.
One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume. 
Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. 
Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison.
A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison. 
DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated.
DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated. Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367 (https://www.neb.com/products/m0367-blunt-ta-ligase-master-mix) ). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232 (https://www.neb.com/products/n3232-1-kb-dna-ladder) ).
Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232).

 

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Monarch® DNA Cleanup Columns (5 μg) T1034-2 25 1 x 50 columns
  Monarch® Collection Tubes (2 ml) T1018-2 25 1 x 50 tubes
  Monarch® DNA Wash Buffer T1032-2 25 1 x 5 ml
  Monarch® Gel Dissolving Buffer T1021-2 25 1 x 47 ml
  Monarch® DNA Elution Buffer T1016-21 25 1 x 3 ml
  Monarch® DNA Cleanup Columns (5 μg) T1034-2 25 5 x 50 columns
  Monarch® Collection Tubes (2 ml) T1018-2 25 5 x 50 tubes
  Monarch® DNA Wash Buffer T1032-3 25 1 x 25 ml
  Monarch® Gel Dissolving Buffer T1021-3 25 1 x 235 ml
  Monarch® DNA Elution Buffer T1016-2 25 1 x 7 ml
Features
  • Elute in as little as 6 μl
  • Prevent buffer retention and salt carry-over with optimized column design
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging

Properties & Usage



Notes
  • The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves and eye protection.
Additional Citations
  • Wang M, Wang B, Jiang K, Liu M, Shi X, Wang L. (2018) A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri Fish Shellfish Immunol PubMedID: 29127027,
  • Kose, S. H., Grice, K., Orsi, W. D., Ballal, M., and Coolen, M. J. L. (2018) Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria Sci Rep
  • Bornikoel J, Staiger J, Madlung J, Forchhammer K, Maldener I. (2018) LytM factor Alr3353 affects filament morphology and cell-cell communication in the multicellular cyanobacterium Anabaena sp. PCC 7120 Mol Microbiol PubMedID: 29437253,
  • Kang B, Peng B, Wu H, Liu L, Wu W, Gu Q. (2018) Host-associated selection of a P3 mutant of zucchini yellow mosaic virus affects viral infectivity in watermelon Arch Virol PubMedID: 29426994,
  • Perniss A, Schmidt N, Gurtner C, Dietert K, Schwengers O, Weigel M, Hempe J, Ewers C, Pfeil U, Gärtner U, Gruber AD, Hain T, Kummer W (2018) Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Sci Rep 8(1):5681, DOI: 10.1038/s41598-018-23830-4
  • Roussy M, Bilodeau M, Jouan L, Tibout P, Laramée L, Lemyre E, Cardin S, Sauvageau C, Couture F, Choblet A, Patey N, Gendron P, Duval M, Teira P, Hébert J, Wilhelm BT, Choi JK, Gruber TA, Bittencourt H, Cellot S. (2018) NUP98-BPTF gene fusion identified in primary refractory acute megakaryoblastic leukemia of infancy Genes Chromosomes Cancer PubMedID: 29427526,
  • Hua Yang, Silvia Jenni, Milena Colovic, Helen Merkens, Carlee Poleschuk, Isabel Rodrigo, Qing Miao, Bruce F. Johnson4, Michael J. Rishel4, Vesna Sossi, Jack M. Webster, François Bénard and Paul Schaffer (2017) F-5-Fluoroaminosuberic Acid as a Potential Tracer to Gauge Oxidative Stress in Breast Cancer Models. J Nucl MedPubMedID: 5331935
  • Zhang Y, Tanner N (2017) Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein Sci RepPubMedID: 28819114
  • Adam Waalkes, Kelsi Penewit, Brent L. Wood, David Wu, and Stephen J. Salipante (2017) Ultrasensitive detection of acute myeloid leukemia minimal residual disease using single molecule molecular inversion probes. HaematologicaPubMedID: 28572161
  • Hannah G. Reich, Deborah L. Robertson, Gretchen Goodbody-Gringley (2016) Do the shuffle: Changes in Symbiodinium consortia throughout juvenile coral development. PLOS OnePubMedID: 28182684
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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