Product Class: Other

Monarch® RNA Cleanup Columns (10 μg)
Item# Description List Price Web Price Qty
T2037L Monarch® RNA Cleanup Columns(10 μg) - 100 columns + collection tubes $280.00
$252.00
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

 

    Product Introduction

    The Monarch RNA Cleanup Columns (10 µg) are a component of the Monarch RNA Cleanup Kit (10 µg, NEB #T2030) and can be used to purify up to 10 µg of RNA from enzymatic reactions. They are supplied with Monarch Collection Tubes II (NEB #T2018). RNA Cleanup Binding Buffer (NEB #T2041) and Monarch RNA Wash Buffer (NEB #T2042) are also available separately.

    • Optimal for cleaning up RNA from a variety of enzymatic reactions
    • Ideal for the concentration of RNA into small volumes (elute in ≥ 6 µl)
    • 70-100% RNA recovery, even with inputs <2 0 ng
    • Purified RNA is ready for use in a wide variety of downstream applications
    • Unique column design prevents buffer carryover and elution of silica particulates

    Learn more about Monarch DNA and RNA purification products

    Product Information

    Description

    The Monarch RNA Cleanup Columns (10 µg) are a component of the Monarch RNA Cleanup Kit (10 µg) (NEB #T2030) and can be used to purify up to 10 µg of RNA from enzymatic reactions. The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 6 μl and is ready for use in a variety of downstream applications including RT-PCR, RNA library prep for NGS, and labelling.

    Monarch RNA Cleanup Columns are also available for 50 µg (NEB #T2047) and 500 µg (NEB #T2057) binding capacities.

    Figure 1: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 6 µl

    rRNA (10 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #2030) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260 as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in as little as 6 µl.
    Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of <20 ng of RNA in as little as 6 µl

    A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A260, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.  
    Product Categories:
    RNA Cleanup Products,
    RNA Reagents Products,
    RNA Extraction & Purification Products
    ,
    Nucleic Acid Purification Products
    Applications:
    RNA Analysis,
    RNA Purification and Isolation

    Properties & Usage

      Protocols, Manuals & Usage

      Protocols

      1. Monarch® RNA Cleanup Kit Protocol
      2. Purification of RNA from the Aqueous Phase Following TRIzol®/Chloroform Extraction using the Monarch® RNA Cleanup Kits
      3. Separation of Large and Small RNA into Fractions using the Monarch® RNA Cleanup Kits
      4. Extraction of RNA from Agarose Gels using the Monarch® RNA Cleanup Kits

      Manuals

      The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

      Usage & Guidelines

      • Avoiding Ribonuclease Contamination
      • Guidelines for RNA Quantitation
      • Guidelines for Working with RNA During RNA Cleanup

      Faqs & Troubleshooting

      FAQs

      1. What is the maximum binding capacity of the Monarch RNA Cleanup Column provided with the Monarch RNA Cleanup Kit?
      2. What is the smallest volume of nuclease-free water that can be used for elution with the Monarch RNA Cleanup Columns?
      3. Can I get better recovery with the Monarch RNA Cleanup Kits if I do a second elution with my eluent from the first elution?
      4. What factors affect my (A260/A230) when using the Monarch RNA Cleanup Kits?
      5. What size RNA can be purified with the Monarch RNA Cleanup Kit?
      6. Can I use the Monarch RNA Cleanup Kit to cleanup up my DNase I-treated RNA?
      7. Can I use the Monarch RNA Cleanup Kits to purify RNA from agarose gels?
      8. Can I use the Monarch RNA Cleanup Kits to cleanup RNA after a TRIzol®/chloroform extraction?
      9. Are the Monarch RNA Cleanup Kits compatible with Luna RT-qPCR reagents?
      10. Are the Monarch RNA Cleanup Kits compatible with NEBNext reagents for RNA library prep?
      11. How can I assess RNA integrity and purity?
      12. Are the columns in the Monarch RNA Cleanup Kits the same as those in the Monarch Total RNA Miniprep Kit (NEB #T2010)?
      13. Can I purchase Monarch® buffers and columns separately?

      Troubleshooting

      • Troubleshooting Guide for RNA Cleanup

      Quality, Safety & Legal

      Quality Assurance Statement

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

      Specifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

      Certificate Of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

      Legal and Disclaimers

      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.